Huang X, Liu Y, Zhang Y
Department of Pathology, Basic Medical Sciences of Fourth Military Medical University, Xi'an.
Zhonghua Yi Xue Za Zhi. 1998 Jan;78(1):40-3.
To determine the effect of the soluble immunocomplexes on the pathogenesis of hemorrhagic fever with renal syndrome (HFRS).
The soluble immunocomplex (IC) consisting of HFRS virus antigen and antibody was prepared previously in vitro, and was intravenously injected into BALB/c mice. The localization of IC in the tissues of mice was investigated by immunohistochemical method ABC.
The results showed that 24 hours after injection, ICs were localized mostly in the blood vessels, such as the liver sinusoids, the splenic sinuses, the blood vessels of the mucosa and submucosa of the intestine, and the capillaries of the renal glomeruli, etc. 48 hours later or more, IC localized primarily on the parachymal cells, e.g., the hepatocytes, the epithelial cells of the renal tubules as well as some epithelial cells of the ducts and alveoli of some glands (submandular glands, pancreas). The cells degenerated in the areas with IC, especially in the hepatocytes and epithelial cells of renal tubules. These lesions were very similar to the pathological changes of the HFRS' patients, and were not found in patients injected with other IC.
The soluble IC of HFRS can cause direct tissue injury in the HFRS.
确定可溶性免疫复合物对肾综合征出血热（HFRS）发病机制的影响。
预先在体外制备由HFRS病毒抗原和抗体组成的可溶性免疫复合物（IC），并将其静脉注射到BALB/c小鼠体内。采用免疫组织化学ABC法研究IC在小鼠组织中的定位。
结果显示，注射后24小时，IC主要定位在血管中，如肝血窦、脾血窦、肠黏膜和黏膜下层的血管以及肾小球毛细血管等。48小时及以后，IC主要定位在实质细胞上，如肝细胞、肾小管上皮细胞以及一些腺体（下颌下腺、胰腺）的导管和肺泡的一些上皮细胞。在有IC的区域细胞发生变性，尤其是肝细胞和肾小管上皮细胞。这些病变与HFRS患者的病理变化非常相似，而在注射其他IC的患者中未发现。
HFRS的可溶性IC可在HFRS中引起直接的组织损伤。
