Liu Y F, Yang S J, Yan P S, Liu Y Y
Department of Pathology, Fourth Military Medical College, Xi'an.
Chin Med J (Engl). 1994 Jun;107(6):444-9.
Using repeated PAP or repeated PAP and ABC immunocytochemical methods, we were able to demonstrate viral antigens, Ig and Clq in the tissues of 20 autopsy materials that had been preserved for 3-30 years. Serial paraffin sections were stained with the first antibodies against both viral antigens (G2 and Np) and human IgG, IgM, C3 and Clq. Immunocomplex, composed of viral antigen, IgG and Clq were found diffusely in the cells of each organ and extracellularly in the sera, various secretions and exudates. When stained by A25 etc, coarse granular antigen or inclusion bodies were found without demonstrable Ig and Clq. It was concluded that the immunocomplex in tissues of epidemic hemorrhagic fever in Shaanxi Province, China was both intracellular and extracellular and was perhaps soluble due to antigens in excess, with characteristics quite different from that of other immune diseases.
采用重复PAP法或重复PAP与ABC免疫细胞化学方法，我们能够在20份保存3至30年的尸检材料组织中显示病毒抗原、免疫球蛋白（Ig）和补体C1q。连续石蜡切片用针对病毒抗原（G2和Np）以及人IgG、IgM、C3和C1q的第一抗体染色。由病毒抗原、IgG和C1q组成的免疫复合物在每个器官的细胞中弥漫性存在，并在血清、各种分泌物和渗出物的细胞外发现。当用A25等染色时，发现有粗大颗粒状抗原或包涵体，但未显示出Ig和C1q。结论是，中国陕西省流行性出血热组织中的免疫复合物既存在于细胞内也存在于细胞外，可能因抗原过量而呈可溶性，其特征与其他免疫疾病有很大不同。
