大肠杆菌dnaB和dnaC(D)基因产物的体外相互作用
Interaction of Escherichia coli dnaB and dnaC(D) gene products in vitro.
作者信息
Wickner S, Hurwitz J
出版信息
Proc Natl Acad Sci U S A. 1975 Mar;72(3):921-5. doi: 10.1073/pnas.72.3.921.
Abstract
Purified E. coli dnaB and dnaC(D) gene products interact physically and functionally in vitro. This interaction was demonstrated as follows: (a) A complex of dnaB and dnaC(D) gene products was isolated by gel filtration; ATP specifically was required for isolation of the complex. (b) The DNA-independent ribonucleoside triphosphatase activity associated with dnaB gene product was inhibited by dnaC(D) gene product. (c) The dnaC(D) gene product was protected from inactivation by N-ethyl-maleimide by the combination of dnaB gene product and ATP; this protection required ATP specifically.
摘要
纯化的大肠杆菌dnaB和dnaC(D)基因产物在体外发生物理和功能相互作用。这种相互作用通过以下方式得到证明：(a) 通过凝胶过滤分离出dnaB和dnaC(D)基因产物的复合物；分离该复合物特别需要ATP。(b) 与dnaB基因产物相关的不依赖DNA的核糖核苷三磷酸酶活性受到dnaC(D)基因产物的抑制。(c) dnaB基因产物和ATP的组合可保护dnaC(D)基因产物不被N-乙基马来酰亚胺灭活；这种保护特别需要ATP。
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