Fogeda M, López-Alcorocho J M, Bartolomé J, Arocena C, Martín M A, Carreño V
Department of Hepatology, Fundación Jiménez Díaz, and Fundación para el Estudio de las Hepatitis Virales, Madrid, Spain.
J Virol. 2000 Sep;74(17):7936-42. doi: 10.1128/jvi.74.17.7936-7942.2000.
To study the existence of GB virus C/hepatitis G virus (GBV-C/HGV) variants with different tropism, we have analyzed the heterogeneity and quasispecies composition of GBV-C/HGV isolated from in vitro-infected peripheral blood mononuclear cells (PBMC) and from sera, livers, and PBMC from two chronically infected patients. For this purpose, the GBV-C/HGV 5' noncoding region (5'NCR) was amplified by reverse transcription-PCR and the amplified products were cloned and sequenced. These analyses showed that the master 5'NCR sequences isolated from the in vitro-infected PBMC and from the PBMC isolated from the patient whose serum was used as the inoculum were identical but different from that of the inoculum. Furthermore, phylogenetic analysis revealed that all PBMC sequences grouped together into a branch which was separate from those of the inoculum. For one of the two chronically infected patients, all the sequences from the PBMC and one from the liver clustered into a single branch while the sequences from the serum and all the other liver sequences grouped together in the other branch. For the other patient, the sequences from the serum and PBMC and three sequences from the liver grouped together into one branch, while the remaining five sequences from the liver were separated in a different cluster. In conclusion, our results support the existence of different GBV-C/HGV variants with different tissue tropism.
为研究具有不同嗜性的GB病毒C/庚型肝炎病毒（GBV-C/HGV）变异体的存在情况，我们分析了从体外感染的外周血单个核细胞（PBMC）以及两名慢性感染患者的血清、肝脏和PBMC中分离出的GBV-C/HGV的异质性和准种组成。为此，通过逆转录聚合酶链反应（RT-PCR）扩增GBV-C/HGV 5'非编码区（5'NCR），并对扩增产物进行克隆和测序。这些分析表明，从体外感染的PBMC以及从以其血清作为接种物的患者体内分离出的PBMC中获得的主要5'NCR序列相同，但与接种物的序列不同。此外，系统发育分析显示，所有PBMC序列聚为一个分支，与接种物的序列分支分开。对于两名慢性感染患者中的一名，PBMC的所有序列以及肝脏的一个序列聚为一个单一分支，而血清的序列以及肝脏的所有其他序列聚为另一个分支。对于另一名患者，血清和PBMC的序列以及肝脏的三个序列聚为一个分支，而肝脏的其余五个序列则分在不同的簇中。总之，我们的结果支持存在具有不同组织嗜性的不同GBV-C/HGV变异体。
