Warden G D, Mason A D, Pruitt B A
Ann Surg. 1975 Mar;181(3):363-9. doi: 10.1097/00000658-197503000-00019.
Polymorphonuclear leukocytes from burned patients exhibit suppressed chemotaxis possibly related to the susceptibility of such patients to opportunistic infection. This study assesses the effect of normal serum upon burn-suppressed leukocytes and the effects of three commonly used topical chemotherapeutic agents upon the chemotaxis exhibited by granulocytes from normal controls. In vitro incubation with normal serum restored chemotaxis to normal in the suppressed granulocytes from burned patients. The serum factor responsible for this restoration was heat labile. Serum albumin alone did not exhibit this effect. Both mafenide and silver sulfadiazine suppressed the chemotactic function of granulocytes obtained from normal controls, while silver nitrate exhibited no such activity. Studies of the chemotactic function of control granulocytes after incubation with sera from burned patients yielded similar results; only the sera from patients treated with silver nitrate failed to suppress normal leukotaxis. The chemotactic impairment found in leukocytes from burned patients, however, while related to burn size and predictive of prognosis, did not vary with the agent used for the topical therapy. These data suggest the presence of a reversible intrinsic defect in leukotaxis consequent to burn injury, related to some factor deficient in burn serum. In addition, extrinsic impairment of normal granulocyte leukotaxis by two commonly used chemotherapeutic agents is demonstrated.
烧伤患者的多形核白细胞表现出趋化性受抑制，这可能与此类患者易发生机会性感染有关。本研究评估了正常血清对烧伤抑制的白细胞的影响，以及三种常用局部化疗药物对正常对照粒细胞趋化性的影响。与正常血清进行体外孵育可使烧伤患者受抑制的粒细胞趋化性恢复正常。负责这种恢复的血清因子对热不稳定。单独的血清白蛋白不表现出这种作用。甲磺灭脓和磺胺嘧啶银均抑制从正常对照获得的粒细胞的趋化功能，而硝酸银则无此活性。对正常对照粒细胞与烧伤患者血清孵育后的趋化功能研究得出了类似结果；只有用硝酸银治疗的患者的血清未能抑制正常白细胞趋化性。然而，烧伤患者白细胞中发现的趋化性损害虽然与烧伤面积有关且可预测预后，但并不因用于局部治疗的药物而异。这些数据表明，烧伤损伤会导致白细胞趋化性存在可逆的内在缺陷，这与烧伤血清中某种缺乏的因子有关。此外，还证明了两种常用化疗药物对正常粒细胞趋化性的外在损害。
