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细菌中的核糖体蛋白S1与多肽链起始

Ribosomal protein S1 and polypeptide chain initiation in bacteria.

作者信息

Szer W, Hermoso J M, Leffler S

出版信息

Proc Natl Acad Sci U S A. 1975 Jun;72(6):2325-9. doi: 10.1073/pnas.72.6.2325.

DOI:10.1073/pnas.72.6.2325
PMID:1094462
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC432750/
Abstract

Among several subspecies of 30S subunits of Escherichia coli observed by polyacrylamide-agarose gel electrophoresis, only the slow-moving, protein S1-containing subspecies participates in the formation of the 30S initiation complex with coliphage MS2 RNA as mRNA; the other subspecies retain activity with AUG as mRNA; they are also active in the poly(U)-directed binding of Phe-tRNA. Protein S1 from Caulobacter crescentus substitutes for E. coli S1 despite the fact that C. crescentus ribosomes do not bind MS2 RNA. Under appropriate conditions, the entire population of E. coli 30S subunits can be isolated as the S1-containing subspecies. Protein S1 is lost by salt treatment of ribosomes.

摘要

通过聚丙烯酰胺-琼脂糖凝胶电泳观察到的大肠杆菌30S亚基的几个亚种中，只有移动较慢、含蛋白质S1的亚种参与与作为mRNA的噬菌体MS2 RNA形成30S起始复合物；其他亚种以AUG作为mRNA时保留活性；它们在聚（U）指导的苯丙氨酰-tRNA结合中也具有活性。尽管新月柄杆菌核糖体不结合MS2 RNA，但来自新月柄杆菌的蛋白质S1可替代大肠杆菌的S1。在适当条件下，大肠杆菌30S亚基的整个群体都可以分离为含S1的亚种。核糖体经盐处理后会失去蛋白质S1。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc1d/432750/1fdd08070dd4/pnas00049-0353-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc1d/432750/57e5d123216d/pnas00049-0352-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc1d/432750/1fdd08070dd4/pnas00049-0353-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc1d/432750/57e5d123216d/pnas00049-0352-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc1d/432750/1fdd08070dd4/pnas00049-0353-a.jpg

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Ribosomal protein S1 and polypeptide chain initiation in bacteria.细菌中的核糖体蛋白S1与多肽链起始
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引用本文的文献

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PLoS One. 2012;7(3):e32702. doi: 10.1371/journal.pone.0032702. Epub 2012 Mar 7.
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S1 ribosomal protein and the interplay between translation and mRNA decay.

本文引用的文献

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The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
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Isolation and properties of crystalline initiation factor F1 from Escherichia coli ribosomes.来自大肠杆菌核糖体的结晶起始因子F1的分离及其性质
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Translation of MS2 RNA by ribosomes from different bacterial species.不同细菌物种的核糖体对MS2 RNA的翻译。
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Primary structure of Escherichia coli ribosomal protein S1 and of its gene rpsA.大肠杆菌核糖体蛋白S1及其基因rpsA的一级结构。
Proc Natl Acad Sci U S A. 1982 Feb;79(4):1008-11. doi: 10.1073/pnas.79.4.1008.
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The binding of ribosomal protein S1 to S1-depleted 30S and 70S ribosomes. A fluorescence anisotropy study of the effects of Mg2+.核糖体蛋白S1与去除S1的30S和70S核糖体的结合。Mg2+作用的荧光各向异性研究。
Nucleic Acids Res. 1983 Aug 25;11(16):5589-602. doi: 10.1093/nar/11.16.5589.
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Targeted random mutagenesis: the use of ambiguously synthesized oligonucleotides to mutagenize sequences immediately 5' of an ATG initiation codon.靶向随机诱变：使用合成不明确的寡核苷酸对紧邻ATG起始密码子5'端的序列进行诱变。
Nucleic Acids Res. 1983 May 25;11(10):3113-21. doi: 10.1093/nar/11.10.3113.
8
Construction of a general vector for efficient expression of mammalian proteins in bacteria: use of a synthetic ribosome binding site.用于在细菌中高效表达哺乳动物蛋白的通用载体构建：合成核糖体结合位点的应用。
Proc Natl Acad Sci U S A. 1981 Sep;78(9):5543-8. doi: 10.1073/pnas.78.9.5543.
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T7 protein synthesis in F-factor-containing cells: evidence for an episomally induced impairment of translation and relation to an alteration in membrane permeability.含有F因子的细胞中T7蛋白质合成：附加体诱导的翻译损伤证据及其与膜通透性改变的关系。
J Virol. 1975 Jan;17(1):94-105. doi: 10.1128/JVI.17.1.94-105.1976.
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9
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Proc Natl Acad Sci U S A. 1974 Sep;71(9):3611-5. doi: 10.1073/pnas.71.9.3611.