Szer W, Hermoso J M, Leffler S
Proc Natl Acad Sci U S A. 1975 Jun;72(6):2325-9. doi: 10.1073/pnas.72.6.2325.
Among several subspecies of 30S subunits of Escherichia coli observed by polyacrylamide-agarose gel electrophoresis, only the slow-moving, protein S1-containing subspecies participates in the formation of the 30S initiation complex with coliphage MS2 RNA as mRNA; the other subspecies retain activity with AUG as mRNA; they are also active in the poly(U)-directed binding of Phe-tRNA. Protein S1 from Caulobacter crescentus substitutes for E. coli S1 despite the fact that C. crescentus ribosomes do not bind MS2 RNA. Under appropriate conditions, the entire population of E. coli 30S subunits can be isolated as the S1-containing subspecies. Protein S1 is lost by salt treatment of ribosomes.
通过聚丙烯酰胺-琼脂糖凝胶电泳观察到的大肠杆菌30S亚基的几个亚种中，只有移动较慢、含蛋白质S1的亚种参与与作为mRNA的噬菌体MS2 RNA形成30S起始复合物；其他亚种以AUG作为mRNA时保留活性；它们在聚（U）指导的苯丙氨酰-tRNA结合中也具有活性。尽管新月柄杆菌核糖体不结合MS2 RNA，但来自新月柄杆菌的蛋白质S1可替代大肠杆菌的S1。在适当条件下，大肠杆菌30S亚基的整个群体都可以分离为含S1的亚种。核糖体经盐处理后会失去蛋白质S1。
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