• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

大肠杆菌核糖体蛋白L1、L11和L16在核糖体亚基缔合及70-S起始复合物形成中的重要性。

The importance of Escherichia coli ribosomal proteins L1, L11 and L16 for the association of ribosomal subunits and the formation of the 70-S initiation complex.

作者信息

Kazemie M

出版信息

Eur J Biochem. 1975 Oct 15;58(2):501-10. doi: 10.1111/j.1432-1033.1975.tb02398.x.

DOI:10.1111/j.1432-1033.1975.tb02398.x
PMID:1102306
Abstract

50-S subunits were washed with LiCl solutions of different concentrations. After washing with 1 M LiCl solution the particles lost their ability to attach either to 30-S subunits or to the AUG - 30-S subunit - fMet-tRNAfMet complex or to a poly(U) - 30-S subunit - Phe-tRNAPhe complex. Those proteins which were removed by LiCl were fractionated on a Sephadex G-100 column. Of the fractionated proteins only the combinations L1 and L11 or L1 and L16 were essential for the association of 50-S 1.0 cores (particles prepared by washing 50-S subunits in 1.0 M LiCl) with 30-S subunits. These three proteins were also required for the formation of a stable complex between 50-S 1.0 cores, mRNA, 30-S subunits and aminoacyl-tRNA.

摘要

用不同浓度的LiCl溶液洗涤50 - S亚基。用1M LiCl溶液洗涤后，颗粒失去了与30 - S亚基、AUG - 30 - S亚基 - fMet - tRNAfMet复合物或聚（U） - 30 - S亚基 - Phe - tRNAPhe复合物结合的能力。那些被LiCl去除的蛋白质在Sephadex G - 100柱上进行分级分离。在分级分离的蛋白质中，只有L1和L11或L1和L16的组合对于50 - S 1.0核心（通过在1.0M LiCl中洗涤50 - S亚基制备的颗粒）与30 - S亚基的结合是必需的。这三种蛋白质也是在50 - S 1.0核心、mRNA、30 - S亚基和氨酰 - tRNA之间形成稳定复合物所必需的。

相似文献

1
The importance of Escherichia coli ribosomal proteins L1, L11 and L16 for the association of ribosomal subunits and the formation of the 70-S initiation complex.大肠杆菌核糖体蛋白L1、L11和L16在核糖体亚基缔合及70-S起始复合物形成中的重要性。
Eur J Biochem. 1975 Oct 15;58(2):501-10. doi: 10.1111/j.1432-1033.1975.tb02398.x.
2
Binding of aminoacyl-tRNA to reconstituted subparticles of Escherichia coli large ribosomal subunits.氨酰tRNA与大肠杆菌大核糖体亚基的重组亚颗粒的结合。
Eur J Biochem. 1976 Aug 16;67(2):373-8. doi: 10.1111/j.1432-1033.1976.tb10701.x.
3
Ribosomal protein L16 binds to the 3'-end of transfer RNA.核糖体蛋白L16与转运RNA的3'末端结合。
FEBS Lett. 1984 Jan 23;166(1):53-6. doi: 10.1016/0014-5793(84)80043-5.
4
A complex between initiation factor IF2, guanosine triphosphate, and fMet-tRNA: an intermediate in initiation complex formation.起始因子IF2、鸟苷三磷酸和甲酰甲硫氨酸转运核糖核酸之间的复合物：起始复合物形成过程中的一个中间体。
Proc Natl Acad Sci U S A. 1971 Dec;68(12):3122-6. doi: 10.1073/pnas.68.12.3122.
5
The binding of Met-tRNAf to isolated 40-S ribosomal subunits and the formation of Met-tRNAf - 80-S-ribosome initiation complexes.甲硫氨酰 - 起始tRNA与分离的40 - S核糖体亚基的结合以及甲硫氨酰 - 起始tRNA - 80 - S核糖体起始复合物的形成。
Eur J Biochem. 1976 Feb 16;62(2):243-55. doi: 10.1111/j.1432-1033.1976.tb10154.x.
6
Structural and functional properties of ribosomes crosslinked with dimethylsuberimidate.与亚胺二甲酯交联的核糖体的结构和功能特性
Proc Natl Acad Sci U S A. 1972 Dec;69(12):3769-73. doi: 10.1073/pnas.69.12.3769.
7
Further evidence for the participation of proteins S 3, S 14 and S 19 in tRNA binding to E. coli 30 S subunits.蛋白质S3、S14和S19参与转运核糖核酸与大肠杆菌30S亚基结合的进一步证据。
Mol Biol Rep. 1976 Nov;3(2):105-11. doi: 10.1007/BF00423223.
8
Ribosomal protein S1 and polypeptide chain initiation in bacteria.细菌中的核糖体蛋白S1与多肽链起始
Proc Natl Acad Sci U S A. 1975 Jun;72(6):2325-9. doi: 10.1073/pnas.72.6.2325.
9
[Protein L16 of the Escherichia coli ribosome: possible role in protein biosynthesis].[大肠杆菌核糖体的蛋白质L16：在蛋白质生物合成中的可能作用]
Mol Biol (Mosk). 1984 Nov-Dec;18(6):1597-605.
10
Formation of a mammalian initiation complex with reovirus messenger RNA, methionyl-tRNA F , and ribosomal subunits.呼肠孤病毒信使核糖核酸、甲硫氨酰 - tRNA F和核糖体亚基形成哺乳动物起始复合物。
Proc Natl Acad Sci U S A. 1972 May;69(5):1234-8. doi: 10.1073/pnas.69.5.1234.

引用本文的文献

1
RbgA ensures the correct timing in the maturation of the 50S subunits functional sites.RbgA 确保了 50S 亚基功能部位成熟的时机正确。
Nucleic Acids Res. 2022 Oct 28;50(19):10801-10816. doi: 10.1093/nar/gkac059.
2
Post-translational hydroxylation by 2OG/Fe(II)-dependent oxygenases as a novel regulatory mechanism in bacteria.2OG/Fe(II) 依赖性加氧酶介导的翻译后羟基化作为细菌中的一种新型调控机制。
Front Microbiol. 2015 Jan 15;5:798. doi: 10.3389/fmicb.2014.00798. eCollection 2014.
3
Defect in the formation of 70S ribosomes caused by lack of ribosomal protein L34 can be suppressed by magnesium.
缺乏核糖体蛋白L34导致的70S核糖体形成缺陷可被镁抑制。
J Bacteriol. 2014 Nov;196(22):3820-30. doi: 10.1128/JB.01896-14. Epub 2014 Sep 2.
4
Functional domains of the 50S subunit mature late in the assembly process.50S 亚基的功能域在组装过程的后期成熟。
Nucleic Acids Res. 2014 Mar;42(5):3419-35. doi: 10.1093/nar/gkt1295. Epub 2013 Dec 13.
5
Cotranscription of two genes necessary for ribosomal protein L11 methylation (prmA) and pantothenate transport (panF) in Escherichia coli K-12.大肠杆菌K-12中核糖体蛋白L11甲基化所需的两个基因（prmA）和泛酸盐转运基因（panF）的共转录。
J Bacteriol. 1993 Nov;175(22):7178-88. doi: 10.1128/jb.175.22.7178-7188.1993.
6
The major transcripts of the kinetoplast DNA of Trypanosoma brucei are very small ribosomal RNAs.布氏锥虫动基体DNA的主要转录本是非常小的核糖体RNA。
Nucleic Acids Res. 1983 Jan 11;11(1):105-25. doi: 10.1093/nar/11.1.105.
7
Properties and regulation of the GTPase activities of elongation factors Tu and G, and of initiation factor 2.延伸因子Tu和G以及起始因子2的GTP酶活性的特性与调控
Mol Cell Biochem. 1981 Mar 27;35(3):129-58. doi: 10.1007/BF02357085.
8
Peptidyltransferase activity of ribosomes and a ribosome precursor from a mutant of Escherichia coli.来自大肠杆菌突变体的核糖体及核糖体前体的肽基转移酶活性
Biochem J. 1976 Dec 15;160(3):721-6. doi: 10.1042/bj1600721.