Montell D J, Rorth P, Spradling A C
Howard Hughes Medical Institute, Carnegie Institution of Washington, Department of Embryology, Baltimore, Maryland 21210.
Cell. 1992 Oct 2;71(1):51-62. doi: 10.1016/0092-8674(92)90265-e.
During Drosophila oogenesis six to ten follicle cells, the border cells, undergo a dramatic and stereotypic migration through the developing egg chamber. We identified four independent P element insertion mutations that specifically blocked border cell migration. They defined a single, novel locus that was named slow border cells (slbo), because hypomorphic alleles caused delayed onset of the migration. Laser ablation of the border cells, or failure of their migration, caused improper morphogenesis of the micropyle, the egg-shell structure through which the sperm enters at fertilization. The slbo locus was found to encode a product homologous to the CCAAT/enhancer-binding protein (C/EBP), a basic region-leucine zipper transcription factor. Drosophila C/EBP may be required for the expression of gene products mediating border cell migration.
在果蝇卵子发生过程中，六到十个卵泡细胞，即边缘细胞，会在发育中的卵室中经历剧烈且刻板的迁移。我们鉴定出四个独立的P因子插入突变，它们特异性地阻断了边缘细胞的迁移。这些突变定义了一个单一的新基因座，被命名为慢边缘细胞（slbo），因为亚效等位基因导致迁移起始延迟。对边缘细胞进行激光消融或其迁移失败，会导致受精孔形态发生异常，受精孔是精子在受精时进入的卵壳结构。发现slbo基因座编码一种与CCAAT/增强子结合蛋白（C/EBP）同源的产物，C/EBP是一种碱性区域-亮氨酸拉链转录因子。果蝇C/EBP可能是介导边缘细胞迁移的基因产物表达所必需的。
