Olut A I, Ertugrul D T, Kocagoz T, Er M, Emri S
Dept of Clinical Microbiology, Hacettepe University, Ankara, Turkey.
Monaldi Arch Chest Dis. 2000 Apr;55(2):110-3.
After the recognition of human herpes virus 8 (HHV-8) in Kaposi's sarcoma lesions, this new virus has been shown to be associated with various types of malignancy. One of them, body cavity-based lymphoma, is a high grade B-cell lymphoma arising from the body cavities. Similarly, mesothelioma is a tumour that originates from the serosal linings of the pleural, pericardial and peritoneal cavities. One of the striking characteristics of mesothelioma cells is the secretion of interleukin-6 (IL-6). Also, it is known that HHV-8 upregulates the levels of IL-6, and this virally originated IL-6 is a well-established growth factor for HHV-8-associated lesions. Therefore, it was hypothesized that HHV-8 may have a role in the pathogenesis of malignant mesothelioma. Twenty-nine pleural biopsy specimens from environmentally induced malignant mesothelioma patients were investigated for the presence of HHV-8 deoxyribonucleic acid (DNA) using the polymerase chain reaction (PCR). Control pleural samples were collected from 15 biopsy specimens from patients with tuberculosis. From all samples, a segment of the beta-globulin gene was amplified in order to make sure that the DNA was extracted properly and did not contain any inhibitors. The specificity of the PCR amplification was confirmed by means of restriction enzyme analysis using Providencia stuartii I. PCR did not reveal HHV-8 DNA in any of the mesothelioma patients or in the control group. It was possible to amplify a segment of the human beta-globulin gene from all the samples of the patient and control groups. HHV-8 DNA was amplified in the control sample, which was a tissue biopsy specimen from a Kaposi's sarcoma lesion, and it was confirmed that the amplified DNA belonged to HHV-8 by restriction enzyme analysis. Malignant mesothelioma continues to be a public health problem in rural parts of Anatolia, Turkey. The major causal factor of the disease is exposure to asbestos and fibrous zeolite (erionite). It seems that there must be some aetiological factors other than exposure to these minerals as not all patients exposed to asbestos develop the disease and the disease is not always associated with any known exposure. From the present study, it was concluded that human herpes virus 8 does not seem to be associated with environmentally induced malignant mesothelioma in Turkey. Other possible causal factors of malignant mesothelioma should be sought.
在卡波西肉瘤病变中发现人类疱疹病毒8（HHV-8）后，这种新病毒已被证明与多种类型的恶性肿瘤有关。其中之一，体腔淋巴瘤，是一种起源于体腔的高级别B细胞淋巴瘤。同样，间皮瘤是一种起源于胸膜、心包膜和腹膜浆膜衬里的肿瘤。间皮瘤细胞的一个显著特征是分泌白细胞介素-6（IL-6）。此外，已知HHV-8会上调IL-6的水平，而这种病毒来源的IL-6是HHV-8相关病变公认的生长因子。因此，有人推测HHV-8可能在恶性间皮瘤的发病机制中起作用。使用聚合酶链反应（PCR）对29例环境诱导性恶性间皮瘤患者的胸膜活检标本进行HHV-8脱氧核糖核酸（DNA）检测。对照胸膜样本取自15例肺结核患者的活检标本。从所有样本中扩增β-球蛋白基因片段，以确保DNA提取正确且不含任何抑制剂。使用斯氏普罗威登斯菌I通过限制性酶切分析确认PCR扩增的特异性。PCR在任何间皮瘤患者或对照组中均未检测到HHV-8 DNA。在患者和对照组的所有样本中均成功扩增出人类β-球蛋白基因片段。在对照样本（一份来自卡波西肉瘤病变的组织活检标本）中扩增出HHV-8 DNA，并通过限制性酶切分析确认扩增的DNA属于HHV-8。在土耳其安纳托利亚农村地区，恶性间皮瘤仍然是一个公共卫生问题。该疾病的主要致病因素是接触石棉和纤维沸石（毛沸石）。似乎除了接触这些矿物质外，肯定还有其他病因，因为并非所有接触石棉的患者都会患病，而且该疾病并不总是与任何已知接触相关。从本研究得出结论，在土耳其，人类疱疹病毒8似乎与环境诱导性恶性间皮瘤无关。应寻找恶性间皮瘤的其他可能致病因素。
