一种新的源自透射电子显微镜（TEM）的超广谱β-内酰胺酶（TEM-91），其ω-环处发生R164C取代，赋予了对头孢他啶的耐药性。
A new TEM-derived extended-spectrum beta-lactamase (TEM-91) with an R164C substitution at the omega-loop confers ceftazidime resistance.
作者信息
Kurokawa Hiroshi, Shibata Naohiro, Doi Yohei, Shibayama Keigo, Kamachi Kazunari, Yagi Tetsuya, Arakawa Yoshichika
机构信息
Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Tokyo, Japan.
出版信息
Antimicrob Agents Chemother. 2003 Sep;47(9):2981-3. doi: 10.1128/AAC.47.9.2981-2983.2003.
Abstract
A new plasmid-mediated TEM-derived extended-spectrum beta-lactamase, TEM-91, was identified in a ceftazidime-resistant (MIC, >128 microg per ml) Escherichia coli strain isolated in 1996 in Japan. TEM-91 has three amino acid substitutions, R164C, M184T, and E240K, compared with TEM-1 penicillinase. The isoelectric point (pI), K(m), and k(cat) of TEM-91 for ceftazidime were 5.7, 179 microM, and 29.0 s(-1), respectively. The K(i) of clavulanic acid for ceftazidime hydrolysis was 30.3 nM.
摘要
1996年在日本分离出的一株对头孢他啶耐药（最低抑菌浓度，>128μg/ml）的大肠杆菌中，鉴定出一种新的质粒介导的源自TEM型的超广谱β-内酰胺酶TEM-91。与TEM-1青霉素酶相比，TEM-91有三个氨基酸取代，即R164C、M184T和E240K。TEM-91对头孢他啶的等电点（pI）、米氏常数（K(m)）和催化常数（k(cat)）分别为5.7、179μM和29.0 s(-1)。棒酸对头孢他啶水解的抑制常数（K(i)）为30.3 nM。
相似文献
1
A new TEM-derived extended-spectrum beta-lactamase (TEM-91) with an R164C substitution at the omega-loop confers ceftazidime resistance.一种新的源自透射电子显微镜（TEM）的超广谱β-内酰胺酶（TEM-91），其ω-环处发生R164C取代，赋予了对头孢他啶的耐药性。
Antimicrob Agents Chemother. 2003 Sep;47(9):2981-3. doi: 10.1128/AAC.47.9.2981-2983.2003.
2
A novel extended-spectrum beta-lactamase CTX-M-23 with a P167T substitution in the active-site omega loop associated with ceftazidime resistance.一种新型的超广谱β-内酰胺酶CTX-M-23，其活性位点ω环发生P167T置换，与头孢他啶耐药相关。
J Antimicrob Chemother. 2004 Aug;54(2):406-9. doi: 10.1093/jac/dkh334. Epub 2004 Jun 16.
3
AmpC beta-lactamase in an Escherichia coli clinical isolate confers resistance to expanded-spectrum cephalosporins.一株大肠埃希菌临床分离株中的AmpCβ-内酰胺酶赋予了对超广谱头孢菌素的耐药性。
Antimicrob Agents Chemother. 2004 Oct;48(10):4050-3. doi: 10.1128/AAC.48.10.4050-4053.2004.
4
Novel SHV-derived extended-spectrum beta-lactamase, SHV-57, that confers resistance to ceftazidime but not cefazolin.新型源自SHV的超广谱β-内酰胺酶SHV-57，它赋予对头孢他啶的耐药性，但对头孢唑林无耐药性。
Antimicrob Agents Chemother. 2005 Feb;49(2):600-5. doi: 10.1128/AAC.49.2.600-605.2005.
5
Molecular genetics of resistance to both ceftazidime and beta-lactam-beta-lactamase inhibitor combinations in Klebsiella pneumoniae and in vivo response to beta-lactam therapy.肺炎克雷伯菌对头孢他啶和β-内酰胺-β-内酰胺酶抑制剂联合用药的耐药分子遗传学及对β-内酰胺治疗的体内反应
J Infect Dis. 1996 Jan;173(1):151-8. doi: 10.1093/infdis/173.1.151.
6
High-level resistance to ceftazidime conferred by a novel enzyme, CTX-M-32, derived from CTX-M-1 through a single Asp240-Gly substitution.一种新型酶CTX-M-32赋予对头孢他啶的高水平耐药性，该酶由CTX-M-1通过单个天冬氨酸240-甘氨酸取代衍生而来。
Antimicrob Agents Chemother. 2004 Jun;48(6):2308-13. doi: 10.1128/AAC.48.6.2308-2313.2004.
7
Mutant TEM beta-lactamase producing resistance to ceftazidime, ampicillins, and beta-lactamase inhibitors.产生对头孢他啶、氨苄西林和β-内酰胺酶抑制剂耐药性的突变体TEMβ-内酰胺酶。
Antimicrob Agents Chemother. 2002 Mar;46(3):646-53. doi: 10.1128/AAC.46.3.646-653.2002.
8
TEM-121, a novel complex mutant of TEM-type beta-lactamase from Enterobacter aerogenes.TEM-121，一种来自产气肠杆菌的新型TEM型β-内酰胺酶复合突变体。
Antimicrob Agents Chemother. 2004 Dec;48(12):4528-31. doi: 10.1128/AAC.48.12.4528-4531.2004.
9
Biochemical and genetic characteristics of TEM-29B, a novel extended spectrum beta-lactamase.新型超广谱β-内酰胺酶TEM-29B的生化及遗传学特征
FEMS Microbiol Lett. 1999 May 1;174(1):185-90. doi: 10.1111/j.1574-6968.1999.tb13567.x.
10
Deciphering the Evolution of Cephalosporin Resistance to Ceftolozane-Tazobactam in Pseudomonas aeruginosa.解析铜绿假单胞菌头孢他啶-他唑巴坦耐药性的演变。
mBio. 2018 Dec 11;9(6):e02085-18. doi: 10.1128/mBio.02085-18.
引用本文的文献
1
Exploring the Role of the Ω-Loop in the Evolution of Ceftazidime Resistance in the PenA β-Lactamase from Burkholderia multivorans, an Important Cystic Fibrosis Pathogen.探究Ω环在嗜麦芽窄食单胞菌（一种重要的囊性纤维化病原体）PenAβ-内酰胺酶头孢他啶耐药性进化中的作用。
Antimicrob Agents Chemother. 2017 Jan 24;61(2). doi: 10.1128/AAC.01941-16. Print 2017 Feb.
2
High adaptability of the omega loop underlies the substrate-spectrum-extension evolution of a class A β-lactamase, PenL.omega 环的高适应性是 PenL 类 Aβ-内酰胺酶的底物谱扩展进化的基础。
Sci Rep. 2016 Nov 9;6:36527. doi: 10.1038/srep36527.
3
Activity of ceftazidime/avibactam against isogenic strains of Escherichia coli containing KPC and SHV β-lactamases with single amino acid substitutions in the Ω-loop.头孢他啶/阿维巴坦对含KPC和SHVβ-内酰胺酶且Ω环有单个氨基酸取代的大肠杆菌同基因菌株的活性。
J Antimicrob Chemother. 2015 Aug;70(8):2279-86. doi: 10.1093/jac/dkv094. Epub 2015 May 8.
4
Pyrosequencing using the single-nucleotide polymorphism protocol for rapid determination of TEM- and SHV-type extended-spectrum beta-lactamases in clinical isolates and identification of the novel beta-lactamase genes blaSHV-48, blaSHV-105, and blaTEM-155.使用单核苷酸多态性协议进行焦磷酸测序，以快速测定临床分离株中TEM型和SHV型超广谱β-内酰胺酶，并鉴定新型β-内酰胺酶基因blaSHV-48、blaSHV-105和blaTEM-155。
Antimicrob Agents Chemother. 2009 Mar;53(3):977-86. doi: 10.1128/AAC.01155-08. Epub 2008 Dec 15.
5
New TEM-derived extended-spectrum beta-lactamase and its genomic context in plasmids from Salmonella enterica serovar derby isolates from Uruguay.来自乌拉圭肠炎沙门氏菌德比分离株质粒的新型透射电镜衍生超广谱β-内酰胺酶及其基因组背景
Antimicrob Agents Chemother. 2006 Feb;50(2):781-4. doi: 10.1128/AAC.50.2.781-784.2006.
6
Practical methods using boronic acid compounds for identification of class C beta-lactamase-producing Klebsiella pneumoniae and Escherichia coli.使用硼酸化合物鉴定产C类β-内酰胺酶肺炎克雷伯菌和大肠埃希菌的实用方法。
J Clin Microbiol. 2005 Jun;43(6):2551-8. doi: 10.1128/JCM.43.6.2551-2558.2005.
7
In vitro and in vivo activities of novel 6-methylidene penems as beta-lactamase inhibitors.新型6-亚甲基青霉烯类作为β-内酰胺酶抑制剂的体外和体内活性
Antimicrob Agents Chemother. 2004 Dec;48(12):4589-96. doi: 10.1128/AAC.48.12.4589-4596.2004.
本文引用的文献
1
Characterization of a novel plasmid-mediated cephalosporinase (CMY-9) and its genetic environment in an Escherichia coli clinical isolate.一株大肠杆菌临床分离株中新型质粒介导的头孢菌素酶（CMY-9）及其遗传环境的特征分析
Antimicrob Agents Chemother. 2002 Aug;46(8):2427-34. doi: 10.1128/AAC.46.8.2427-2434.2002.
2
Characterization of a new extended-spectrum beta-lactamase (TEM-87) isolated in Proteus mirabilis during an Italian survey.在一项意大利调查中，奇异变形杆菌分离出的一种新型超广谱β-内酰胺酶（TEM-87）的特性分析。
Antimicrob Agents Chemother. 2002 Mar;46(3):925-8. doi: 10.1128/AAC.46.3.925-928.2002.
3
Updated sequence information and proposed nomenclature for bla(TEM) genes and their promoters.bla(TEM)基因及其启动子的更新序列信息和建议命名法。
Antimicrob Agents Chemother. 2000 Nov;44(11):3232-4. doi: 10.1128/AAC.44.11.3232-3234.2000.
4
New approaches in the treatment of bacterial infections.
Curr Opin Chem Biol. 2000 Aug;4(4):433-9. doi: 10.1016/s1367-5931(00)00106-x.
5
TEM-72, a new extended-spectrum beta-lactamase detected in Proteus mirabilis and Morganella morganii in Italy.TEM-72，一种在意大利奇异变形杆菌和摩根摩根菌中检测到的新型超广谱β-内酰胺酶。
Antimicrob Agents Chemother. 2000 Sep;44(9):2537-9. doi: 10.1128/AAC.44.9.2537-2539.2000.
6
A new SHV-derived extended-spectrum beta-lactamase (SHV-24) that hydrolyzes ceftazidime through a single-amino-acid substitution (D179G) in the -loop.一种新的源自SHV的超广谱β-内酰胺酶（SHV-24），它通过β-环中的单个氨基酸取代（D179G）水解头孢他啶。
Antimicrob Agents Chemother. 2000 Jun;44(6):1725-7. doi: 10.1128/AAC.44.6.1725-1727.2000.
7
A preliminary survey of extended-spectrum beta-lactamases (ESBLs) in clinical isolates of Klebsiella pneumoniae and Escherichia coli in Japan.
FEMS Microbiol Lett. 2000 Mar 1;184(1):53-6. doi: 10.1111/j.1574-6968.2000.tb08989.x.
8
beta-Lactamases of increasing clinical importance.临床重要性日益增加的β-内酰胺酶。
Curr Pharm Des. 1999 Nov;5(11):839-45.
9
Worldwide proliferation of carbapenem-resistant gram-negative bacteria.耐碳青霉烯类革兰氏阴性菌在全球范围内的扩散。
Lancet. 1999 Sep 11;354(9182):955. doi: 10.1016/S0140-6736(05)75707-X.
10
Effects on substrate profile by mutational substitutions at positions 164 and 179 of the class A TEM(pUC19) beta-lactamase from Escherichia coli.大肠杆菌A类TEM（pUC19）β-内酰胺酶第164位和第179位突变取代对底物谱的影响。
J Biol Chem. 1999 Aug 13;274(33):23052-60. doi: 10.1074/jbc.274.33.23052.
Zotero 插件