Farr A G, Kiely J M, Unanue E R
J Immunol. 1979 Jun;122(6):2395-404.
This study reports on the in vitro interactions between T cells from Listeria-immunized mice, macrophages from normal mice, and heat-killed Listeria organisms. This interaction was assayed either by determing the amount of thymocyte mitogen in culture fluids after 24 hr, or by estimating the degree of T cell proliferation after 96 hr. Each assay depended on critical concentrations of macrophages, T cells, and heat-killed Listeria, points that were evaluated in a number of experiments. Both assays required specific Listeria-immune T cells. For an effective interaction, the T cells and the macrophages had to share the I-A region of the H-2 gene complex. Macrophages bearing Ia, which represented a minor population of macrophages, were essential for the proliferative response to macrophage-associated Listeria. Also, Ia-bearing macrophages were an important component in the interactions leading to increased secretion of mitogen. The immunogenic moiety associated with Listeria was short-lived, disappearing 24 hr after uptake of Listeria by macrophages. The interactions were not blocked by anti-Listeria antibodies but were partially sensitive to trypsinization.
本研究报告了来自经李斯特菌免疫小鼠的T细胞、正常小鼠的巨噬细胞以及热灭活李斯特菌之间的体外相互作用。这种相互作用通过测定24小时后培养液中胸腺细胞促有丝分裂原的量，或通过估计96小时后T细胞增殖程度来检测。每种检测都依赖于巨噬细胞、T细胞和热灭活李斯特菌的临界浓度，这些要点在许多实验中进行了评估。两种检测都需要特定的李斯特菌免疫T细胞。为了实现有效的相互作用，T细胞和巨噬细胞必须共享H-2基因复合体的I-A区域。携带Ia的巨噬细胞（代表巨噬细胞的一小部分群体）对于对巨噬细胞相关李斯特菌的增殖反应至关重要。此外，携带Ia的巨噬细胞是导致促有丝分裂原分泌增加的相互作用中的一个重要组成部分。与李斯特菌相关的免疫原性部分寿命较短，在巨噬细胞摄取李斯特菌后24小时消失。这些相互作用未被抗李斯特菌抗体阻断，但对胰蛋白酶处理部分敏感。
