从兔梅毒病灶中分离和纯化梅毒螺旋体。
Isolation and purification of Treponema pallidum from syphilitic lesions in rabbits.
作者信息
Hardy P H, Nell E E
出版信息
Infect Immun. 1975 Jun;11(6):1296-9. doi: 10.1128/iai.11.6.1296-1299.1975.
Abstract
Treponema pallidum were extracted from testicular syphilomas of corticosteroid-treated rabbits and purified by differential centrifugation. The steroid therapy allowed a longer holding time for infected rabbits, which produced greater treponeme yields, averaging 1.58 x 10(10) treponemes per rabbit. The treatment, which also diminished cellular infiltration and increased the extracellular mucoid material in lesions, produced much cleaner suspensions than preparations from nontreated animals. Most of the treponemes in the purified suspensions were still motile, and none carried demonstrable host immunoglobulin. The preparations were free of recognizable host tissue debris and they contained, on the average, 1.9 x 10(-7) mug of protein per treponeme.
摘要
从经皮质类固醇治疗的兔睾丸梅毒瘤中提取梅毒螺旋体，并通过差速离心法进行纯化。类固醇疗法使受感染兔子的存活时间延长，从而产生了更高的螺旋体产量，每只兔子平均产生1.58×10¹⁰个螺旋体。该治疗还减少了病变中的细胞浸润并增加了细胞外黏液物质，与未治疗动物的制剂相比，产生的悬浮液更纯净。纯化悬浮液中的大多数螺旋体仍具有运动能力，且没有携带可检测到的宿主免疫球蛋白。这些制剂没有可识别的宿主组织碎片，平均每个螺旋体含有1.9×10⁻⁷微克蛋白质。
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