van der Sluis J J, ten Kate F J, Vuzevski V D, Stolz E
Department of Dermatovenereology, Erasmus University, Rotterdam, The Netherlands.
Genitourin Med. 1987 Oct;63(5):297-304. doi: 10.1136/sti.63.5.297.
The mucopolysaccharide nature of the material deposited in rabbit testes infected with Treponema pallidum was confirmed by histochemical staining with alcian blue. Differential staining of mucopolysaccharides showed the presence of sulphated mucopolysaccharides as an almost constant feature, whereas in little more than half of the orchitic testes studied variable deposits of hyaluronic acid were seen. The treponemes were almost exclusively present in the areas rich in mucopolysaccharide. A combination staining with the Warthin-Starry method and alcian blue showed treponemes in close association with pre-existing fibrils and cells contained in these fibrils. The latter findings were confirmed by electron microscopy, and the fibroblasts to which treponemes adhered displayed the characteristics of activated cells. The close parallel between the histopathological changes observed here and their descriptions in published reports shows that our specific strain still behaves the same as the original Nichols pathogenic strain of T pallidum.
用阿尔辛蓝进行组织化学染色，证实了感染梅毒螺旋体的兔睾丸中沉积物质的黏多糖性质。黏多糖的鉴别染色显示，硫酸化黏多糖几乎是一个恒定特征，而在研究的略多于一半的睾丸炎睾丸中，可见透明质酸的可变沉积物。梅毒螺旋体几乎只存在于富含黏多糖的区域。用沃辛-斯塔里法和阿尔辛蓝联合染色显示，梅毒螺旋体与预先存在的纤维以及这些纤维中的细胞紧密相关。电子显微镜证实了后者的发现，黏附梅毒螺旋体的成纤维细胞表现出活化细胞的特征。此处观察到的组织病理学变化与其在已发表报告中的描述之间的密切相似性表明，我们的特定菌株仍表现得与梅毒螺旋体原始的致病性尼科尔斯菌株相同。
