Adler P N, Taylor J, Charlton J
Biology Department and Cancer Center, University of Virginia, Charlottesville 22903, USA.
Mech Dev. 2000 Sep;96(2):197-207. doi: 10.1016/s0925-4773(00)00392-0.
The frizzled (fz) gene is required for the development of distally pointing hairs on the Drosophila wing. It has been suggested that fz is needed for the propagation of a signal along the proximal distal axis of the wing. The directional domineering non-autonomy of fz clones could be a consequence of a failure in the propagation of this signal. We have tested this hypothesis in two ways. In one set of experiments we used the domineering non-autonomy of fz and Vang Gogh (Vang) clones to assess the direction of planar polarity signaling in the wing. prickle (pk) mutations alter wing hair polarity in a cell autonomous way, so pk cannot be altering a global polarity signal. However, we found that pk mutations altered the direction of the domineering non-autonomy of fz and Vang clones, arguing that this domineering non-autonomy is not due to an alteration in a global signal. In a second series of experiments we ablated cells in the pupal wing. We found that a lack of cells that could be propagating a long-range signal did not alter hair polarity. We suggest that fz and Vang clones result in altered levels of a locally acting signal and the domineering non-autonomy results from wild-type cells responding to this abnormal signal.
卷曲蛋白（fz）基因对于果蝇翅膀上向远端生长的刚毛的发育是必需的。有人提出，fz对于信号沿翅膀近端-远端轴的传播是必需的。fz克隆的定向性显性非自主性可能是该信号传播失败的结果。我们通过两种方式检验了这一假设。在一组实验中，我们利用fz和梵高（Vang）克隆的显性非自主性来评估翅膀中平面极性信号的方向。棘状蛋白（pk）突变以细胞自主性方式改变翅膀刚毛极性，因此pk不可能改变全局极性信号。然而，我们发现pk突变改变了fz和Vang克隆的显性非自主性方向，这表明这种显性非自主性并非由于全局信号的改变。在第二组实验中，我们去除了蛹期翅膀中的细胞。我们发现，缺乏能够传播长距离信号的细胞并不会改变刚毛极性。我们认为，fz和Vang克隆导致局部作用信号水平改变，而显性非自主性是野生型细胞对这种异常信号作出反应的结果。
