Taylor J, Abramova N, Charlton J, Adler P N
Biology Department and Cancer Center, University of Virginia, Charlottesville, Virginia 22903, USA.
Genetics. 1998 Sep;150(1):199-210. doi: 10.1093/genetics/150.1.199.
Mutations in the Van Gogh gene result in the altered polarity of adult Drosophila cuticular structures. On the wing, Van Gogh mutations cause an altered polarity pattern that is typical of mutations that inactivate the frizzled signaling/signal transduction pathway. The phenotype however, differs from those seen previously, as the number of wing cells forming more than one hair is intermediate between that seen previously for typical frizzled-like or inturned-like mutations. Consistent with Van Gogh being involved in the function of the frizzled signaling/signal transduction pathway, Van Gogh mutations show strong interactions with mutations in frizzled and prickle. Mitotic clones of Van Gogh display domineering cell nonautonomy. In contrast to frizzled clones, Van Gogh clones alter the polarity of cells proximal (and in part anterior and posterior) but not distal to the clone. In further contrast to frizzled clones, Van Gogh clones cause neighboring wild-type hairs to point away from rather than toward the clone. This anti-frizzled type of domineering nonautonomy and the strong genetic interactions seen between frizzled and Van Gogh suggested the possibility that Van Gogh was required for the noncell autonomous function of frizzled. As a test of this possibility we induced frizzled clones in a Van Gogh mutant background and Van Gogh clones in a frizzled mutant background. In both cases the domineering nonautonomy was suppressed consistent with Van Gogh being essential for frizzled signaling.
梵高基因的突变会导致成年果蝇表皮结构的极性改变。在翅膀上，梵高基因突变会导致极性模式改变，这是典型的使卷曲信号/信号转导途径失活的突变所具有的特征。然而，该表型与之前观察到的不同，因为形成不止一根刚毛的翅膀细胞数量介于典型的卷曲样或内翻样突变之前观察到的数量之间。与梵高基因参与卷曲信号/信号转导途径的功能一致，梵高基因突变与卷曲基因和刺基因的突变表现出强烈的相互作用。梵高基因的有丝分裂克隆表现出支配性细胞非自主性。与卷曲基因克隆不同，梵高基因克隆会改变克隆近端（部分还有前后端）细胞的极性，但不会改变克隆远端细胞的极性。与卷曲基因克隆进一步不同的是，梵高基因克隆会使相邻的野生型刚毛指向远离克隆的方向，而不是指向克隆。这种反卷曲型的支配性非自主性以及卷曲基因和梵高基因之间强烈的遗传相互作用表明，梵高基因可能是卷曲基因非细胞自主功能所必需的。为了验证这种可能性，我们在梵高基因突变背景下诱导卷曲基因克隆，在卷曲基因突变背景下诱导梵高基因克隆。在这两种情况下，支配性非自主性都被抑制了，这与梵高基因对卷曲信号至关重要一致。
