核酸酶S1对转运RNA的特异性切割
Specific cleavage of tRNA by nuclease S1.
作者信息
Harada F, Dahlberg J E
出版信息
Nucleic Acids Res. 1975 Jun;2(6):865-71. doi: 10.1093/nar/2.6.865.
Abstract
Nuclease S1 specifically hydrolizes tRNAs in their anticodon loops, forming new 5' phosphate and 3' OH ends. Some single-stranded regions are not cut by nuclease S1. The strong preference of nuclease S1 for the anticodon region can be used for rapid identification of an anticodon-containing oligonucleotide and subsequent identification of the probable amino acid specificity of tRNA.
摘要
核酸酶S1能特异性地水解tRNA反密码子环中的tRNA，形成新的5'磷酸末端和3'羟基末端。一些单链区域不会被核酸酶S1切割。核酸酶S1对反密码子区域的强烈偏好可用于快速鉴定含反密码子的寡核苷酸，并随后鉴定tRNA可能的氨基酸特异性。
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