Berry J A, Biedlingmaier J F, Whelan P J
Division of Otolaryngology-Head and Neck Surgery, Department of Surgery, University of Maryland Medical System, Baltimore, USA.
Otolaryngol Head Neck Surg. 2000 Sep;123(3):246-51. doi: 10.1067/mhn.2000.107458.
Bacterial biofilm formation has been implicated in persistent posttympanostomy otorrhea and irreversible tube contamination. The use of a tympanostomy tube with a resistance to biofilm formation by the most common organisms associated with persistent infection may decrease the incidence of chronic otorrhea and the need for tube removal. In this investigation, scanning electron microscopy was used to compare a phosphorylcholine-coated fluoroplastic tympanostomy tube to plain fluoroplastic and silver oxide-impregnated fluoroplastic for resistance to biofilm formation after in vitro incubation with Staphylococcus aureus or Pseudomonas aeruginosa. Only a biofilm from Pseudomonas formed on the untreated fluoroplastic tubes, whereas the silver oxide-impregnated tubes developed biofilms from both S aureus and P aeruginosa. In contrast, the coated fluoroplastic tube showed resistance to both staphylococcal and pseudomonal biofilm adhesion. This is the first study to demonstrate the effect of a surface treatment of fluoroplastic as a method to inhibit biofilm formation by both S aureus and P aeruginosa. This reinforces our previous studies showing that surface-adherence properties such as charge or slickness or both may be more beneficial than antibacterial treatments in preventing film adhesion.
细菌生物膜形成与鼓膜切开术后持续性耳漏及不可逆转的置管污染有关。使用对与持续性感染相关的最常见生物体形成生物膜具有抗性的鼓膜造孔管，可能会降低慢性耳漏的发生率以及取出置管的必要性。在本研究中，使用扫描电子显微镜将涂有磷酸胆碱的氟塑料鼓膜造孔管与普通氟塑料以及浸渍氧化银的氟塑料进行比较，观察它们在体外与金黄色葡萄球菌或铜绿假单胞菌孵育后形成生物膜的抗性。在未经处理的氟塑料置管上仅形成了来自铜绿假单胞菌的生物膜，而浸渍氧化银的置管上则形成了来自金黄色葡萄球菌和铜绿假单胞菌的生物膜。相比之下，涂覆氟塑料置管对葡萄球菌和假单胞菌生物膜的黏附均表现出抗性。这是第一项证明氟塑料表面处理作为一种抑制金黄色葡萄球菌和铜绿假单胞菌形成生物膜的方法所产生效果的研究。这强化了我们之前的研究，即电荷或光滑度或两者兼具等表面黏附特性在防止生物膜黏附方面可能比抗菌处理更有益。
