Strunz T, Oroszlan K, Schumakovitch I, Güntherodt H, Hegner M
Department of Physics and Astronomy, University of Basel, 4056 Basel, Switzerland.
Biophys J. 2000 Sep;79(3):1206-12. doi: 10.1016/s0006-3495(00)76375-2.
We discuss models for the force-induced dissociation of a ligand-receptor bond, occurring in the context of cell adhesion or single molecule unbinding force measurements. We consider a bond with a structured energy landscape which is modeled by a network of force dependent transition rates between intermediate states. The behavior of a model with only one intermediate state and a model describing a molecular zipper is studied. We calculate the bond lifetime as a function of an applied force and unbinding forces under an increasing applied load and determine the relationship between both quantities. The dissociation via an intermediate state can lead to distinct functional relations of the bond lifetime on force. One possibility is the occurrence of three force regimes where the lifetime of the bond is determined by different transitions within the energy landscape. This case can be related to recent experimental observations of the force-induced dissociation of single avidin-biotin bonds.
我们讨论了在细胞黏附或单分子解链力测量背景下发生的配体-受体键力诱导解离模型。我们考虑了具有结构化能量景观的键，该能量景观由中间状态之间与力相关的跃迁速率网络建模。研究了仅具有一个中间状态的模型和描述分子拉链的模型的行为。我们计算了键寿命作为施加力的函数以及在增加的施加载荷下的解链力，并确定了这两个量之间的关系。通过中间状态的解离可导致键寿命与力之间不同的函数关系。一种可能性是出现三种力状态，其中键的寿命由能量景观内的不同跃迁决定。这种情况可能与最近关于单抗生物素蛋白-生物素键力诱导解离的实验观察结果相关。
