Dubovsky J, Meyer R D
Clin Chim Acta. 1975 Jul 23;62(2):277-86. doi: 10.1016/0009-8981(75)90237-5.
Hydroxyproline-containing polypeptides were isolated from urine by filtration on polyacrylamide gel Bio-Gel P-2. This fraction was quantitatively and qualitatively similar to the nondialysable hydroxyproline in urines of normal adult subjects, patients with chronic uremia, and with primary or secondary hyperparathyroidism. Hydroxyproline polypeptides isolated from urines of patients with extensive Paget's disease or from normal growing subjects contained a group of smaller polypeptides slightly retarded on Bio-Gel P-2 which were not retained by the dialysis membrane Spectrapor-TM. In these two groups of subjects the values of hydroxyproline polypeptides were 35-45 percent higher than those of dialysable hydroxyproline. Increased excretion of hydroxyproline polypeptides was found in chronic uremia, primary and secondary hyperparathyroidism, Paget's bone disease and in normal growing subjects. With the notable exception of chronic uremia the excretion of hydroxyproline polypeptides paralleled that of total and oligopeptidic hydroxyproline. In some cases of chronic uremia the polypeptidic hydroxyproline represented the predominant fraction. On long columns of Bio-Gel P-6 hydroxyproline polypeptides separated into several peaks. The chromatographic pattern was rather characteristic for chronic uremia, secondary hyperparathyroidism, Paget's bone disease and physiological growth. Ahydroxyproline fraction of similar chromatographic properties was isolated from blood serum after deproteinisation with heat or ethanol at pH 4.8. High serum levels of this hydroxyproline fraction were observed in pathological conditions with increased urinary excretion of polypeptidic and nondialysable hydroxyproline.
通过在聚丙烯酰胺凝胶Bio - Gel P - 2上过滤从尿液中分离出含羟脯氨酸的多肽。该组分在定量和定性上与正常成年受试者、慢性尿毒症患者以及原发性或继发性甲状旁腺功能亢进患者尿液中的非透析性羟脯氨酸相似。从患有广泛性佩吉特病的患者尿液或正常生长受试者尿液中分离出的羟脯氨酸多肽含有一组在Bio - Gel P - 2上迁移稍慢的较小多肽，这些多肽不能被透析膜Spectrapor - TM保留。在这两组受试者中，羟脯氨酸多肽的值比可透析性羟脯氨酸的值高35 - 45%。在慢性尿毒症、原发性和继发性甲状旁腺功能亢进、佩吉特骨病以及正常生长受试者中发现羟脯氨酸多肽排泄增加。除慢性尿毒症外，羟脯氨酸多肽的排泄与总羟脯氨酸和寡肽羟脯氨酸的排泄平行。在某些慢性尿毒症病例中，多肽性羟脯氨酸占主要部分。在Bio - Gel P - 6长柱上，羟脯氨酸多肽分离成几个峰。色谱图对于慢性尿毒症、继发性甲状旁腺功能亢进、佩吉特骨病和生理性生长具有相当的特征性。在用热或pH 4.8的乙醇进行脱蛋白后，从血清中分离出具有相似色谱性质的一种羟脯氨酸组分。在多肽性和非透析性羟脯氨酸尿排泄增加的病理状态下，观察到该羟脯氨酸组分的血清水平升高。
