Dubovsky J, Geary W T, Chilcutt D A
Clin Chim Acta. 1977 Apr 1;76(1):41-53. doi: 10.1016/0009-8981(77)90117-6.
Free hydroxylysine and hydroxylysing glycosides were separated from urine and serum extracts on cation exchange resin and assayed spectrophotometrically. The method in conjunction with gel filtration in Bio-Gel P2 allowed to separate from urine also polypeptide hydroxylysine and hydroxylysine bound in small molecules of neutral or acidic character. Glycosylgalactosylhydroxylysineand galactosylhydroxylysine were separated by partition and/or ion exchange chromatography. Patients with chronic renal insufficiency had elevated serum levels and urinary excretion of hydroxylysine glycosides with increased excretion of hydroxylysine bound in polypeptides and in small molecules of neutral or acidic character. The excretion of free hydroxylysine was often within normal limits. When compared to values found in normal growing subjects and in adult patients with increased bone turnover and normal renin function the urinary excretion of hydroxylysine glycosides in chronic uremia was more markedly increased than excretion of hydroxyproline polypeptides and total hydroxyproline.
采用阳离子交换树脂从尿液和血清提取物中分离出游离羟赖氨酸和羟赖氨酸糖苷，并通过分光光度法进行测定。该方法结合Bio-Gel P2凝胶过滤，还能从尿液中分离出多肽羟赖氨酸以及与中性或酸性小分子结合的羟赖氨酸。糖基半乳糖基羟赖氨酸和半乳糖基羟赖氨酸通过分配色谱法和/或离子交换色谱法进行分离。慢性肾功能不全患者血清中羟赖氨酸糖苷水平升高，尿中排泄增加，多肽以及中性或酸性小分子中结合的羟赖氨酸排泄也增加。游离羟赖氨酸的排泄通常在正常范围内。与正常生长的受试者以及骨转换增加且肾素功能正常的成年患者相比，慢性尿毒症患者尿中羟赖氨酸糖苷的排泄比羟脯氨酸多肽和总羟脯氨酸的排泄增加更为明显。
