Dutech C, Maggia L, Joly H I
Laboratoire de Génétique et d'Ecologie Moléculaire, Silvolab-Guyane, Cirad-Forêt, BP 701, 97387 Kourou cedex, France.
Mol Ecol. 2000 Sep;9(9):1427-32. doi: 10.1046/j.1365-294x.2000.01027.x.
The chloroplast genome has been widely used to describe genetic diversity in plant species. Its maternal inheritance in numerous angiosperm species and low mutation rate are suitable characters when inferring historical events such as possible recolonization routes. Here we have studied chloroplast DNA variation using PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) with seven pairs of primers and four restriction enzymes in 14 populations of Vouacapoua americana (Caesalpiniaceae) a neotropical tree sampled throughout French Guiana. Population diversity (Hs), total gene diversity (Ht) and differentiation among populations (GST) were estimated using Nei's method as 0.09, 0.87 and 0.89, respectively. This is consistent with the limited gene flow associated with synzoochory in this species. The genetic structure observed in the north of French Guiana suggests that historical events such as contractions and recent recolonizations have had a large impact on the distribution of genetic diversity in this species.
叶绿体基因组已被广泛用于描述植物物种的遗传多样性。在推断诸如可能的重新定殖路线等历史事件时，其在众多被子植物物种中的母系遗传和低突变率是合适的特征。在此，我们使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP），用七对引物和四种限制性内切酶，研究了法属圭亚那各地采样的新热带树种美洲沃卡豆（Caesalpiniaceae）14个种群的叶绿体DNA变异。使用内氏方法估计种群多样性（Hs）、总基因多样性（Ht）和种群间分化（GST）分别为0.09、0.87和0.89。这与该物种中与同域传播相关的有限基因流一致。在法属圭亚那北部观察到的遗传结构表明，诸如收缩和近期重新定殖等历史事件对该物种遗传多样性的分布产生了重大影响。
