S Emy Koestanti, Misaco Wiwik, Chusniati Sri, Maslachah Lilik
Departmen of Animal Husbandry, Faculty of Veterinary Medicine, Universitas Airlangga, C Campus UNAIR, Jl. Mulyorejo Surabaya 60115 Indonesia.
Departement Clinic Veteriner, Faculty of Veterinary Medicine, Universitas Airlangga, C Campus UNAIR, Jl. Mulyorejo Surabaya 60115 Indonesia.
Afr J Infect Dis. 2018 Mar 7;12(1 Suppl):148-151. doi: 10.2101/Ajid.12v1S.22. eCollection 2018.
Brucellosis in pigs at East Java Indonesia has not only cause great economic losses due to a decrease in productivity of livestock but also are zoonotic. Infection on free brucelosis pigs were initially begun with the infected pigs both male and female, or the use of superior male pigs together. The elimination of the disease either on a group or population is considered as the most effective way to prevent the spread of the disease in pigs. Prevention efforts mainly addressed to vaccination, sanitary maintenace and government policy. The purpose of this study was to isolated and identified as the causative agent.
The survey area were the pig farm owned by breeder farmers in the area of East Java Indonesia, at Kediri, Malang, Blitar and Probolinggo district. Blood samples obtained were tested with RBT. Pigs are suspected of being infected with Brucella if the RBT was positive that characterized with agglutination in the test results. If RBT was positive, bacteriological examination will be performed, with samples of visceral foetus organ, ie liver, spleen, placenta and amniotic fluid. Isolation and identification of were used Brucella Broth and Brucella Agar, and if the bacteri growthwill be continued with biochemical test ie H2S, urease, citrate, catalase and oxidase test. The positive results of showed positive urease, catalase andoxidase, but negative for citrate and H2S.
RBT and bacteriolgical examination showed that 1 sample was positive , and 19 negative. The positive results showed positive urease, catalase and oxidase, but negative for citrate and H2S.
Based on RBT test and bacteriological examination, there was 1 positive sample of brucellla suis, that is sample coming from Kediri district.
印度尼西亚东爪哇省猪的布鲁氏菌病不仅因牲畜生产力下降造成巨大经济损失，而且具有人畜共患性。自由放养的猪感染布鲁氏菌病最初始于感染的公猪和母猪，或者同时使用优良公猪。在群体或种群中消除该疾病被认为是预防猪群中疾病传播的最有效方法。预防措施主要涉及疫苗接种、卫生维护和政府政策。本研究的目的是分离并鉴定出病原体。
调查区域为印度尼西亚东爪哇省凯迪里、玛琅、勿里达和外南梦地区养殖户拥有的猪场。采集的血样用虎红平板凝集试验（RBT）检测。如果RBT检测结果呈阳性（以试验结果中的凝集为特征），则猪被怀疑感染布鲁氏菌。如果RBT呈阳性，将进行细菌学检查，采集内脏胎儿器官样本，即肝脏、脾脏、胎盘和羊水。使用布鲁氏菌肉汤和布鲁氏菌琼脂进行分离和鉴定，如果细菌生长，将继续进行生化试验，即硫化氢（H2S）、尿素酶、柠檬酸盐、过氧化氢酶和氧化酶试验。阳性结果显示尿素酶、过氧化氢酶和氧化酶呈阳性，但柠檬酸盐和H2S呈阴性。
RBT和细菌学检查表明，1份样本布鲁氏菌检测呈阳性，19份呈阴性。阳性结果显示尿素酶、过氧化氢酶和氧化酶呈阳性，但柠檬酸盐和H2S呈阴性。
基于RBT试验和细菌学检查，有1份猪布鲁氏菌阳性样本，即来自凯迪里地区的样本。
