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温和型芽孢杆菌噬菌体SP16的基因组是环状排列且末端冗余的。

Temperate Bacillus bacteriophage SP16 genome is circularly permuted and terminally redundant.

作者信息

Parker A P, Dean D H

出版信息

J Bacteriol. 1986 Aug;167(2):719-21. doi: 10.1128/jb.167.2.719-721.1986.

DOI:10.1128/jb.167.2.719-721.1986
PMID:3015889
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC212950/
Abstract

The physical nature of temperate Bacillus bacteriophage SP16 DNA was analyzed by electron microscopy, exonuclease digestion, denaturation-renaturation experiments, and restriction enzyme analysis. The SP16 genome is a linear molecule 60.0 +/- 2.0 kilobases in length without cohesive ends. Electron micrographs of denatured and renatured SP16 DNA showed that the DNA is circularly permuted. The genome possesses terminal redundancy, as demonstrated by electron microscopy of exonuclease III-digested DNA.

摘要

通过电子显微镜、核酸外切酶消化、变性-复性实验以及限制性内切酶分析，对温和型芽孢杆菌噬菌体SP16 DNA的物理性质进行了分析。SP16基因组是一个线性分子，长度为60.0±2.0千碱基对，没有粘性末端。变性和复性后的SP16 DNA的电子显微镜照片显示，该DNA是环状排列的。如核酸外切酶III消化后的DNA的电子显微镜所示，基因组具有末端冗余。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/13d3/212950/01892017705e/jbacter00207-0300-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/13d3/212950/76d6554963da/jbacter00207-0299-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/13d3/212950/01892017705e/jbacter00207-0300-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/13d3/212950/76d6554963da/jbacter00207-0299-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/13d3/212950/01892017705e/jbacter00207-0300-a.jpg

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本文引用的文献

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Genome of Bacillus subtilis Bacteriophage SPP1: Structure and Nucleotide Sequence of pac, the Origin of DNA Packaging.枯草芽孢杆菌噬菌体SPP1的基因组：DNA包装起始位点pac的结构与核苷酸序列
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Transduction in Bacillus subtilis.枯草芽孢杆菌中的转导
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Similarity of genetic distance determined from DNA thermal denaturation profiles to standard estimates of bacteriophage relatedness.
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The nucleotide sequence of bacteriophage phiX174.噬菌体φX174的核苷酸序列。
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