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使用不同接种物对两种沙门氏菌增菌培养基进行质量控制测试。

Quality control tests of two salmonella enrichment media using different inocula.

作者信息

Harvey R W, Price T H

出版信息

J Hyg (Lond). 1975 Jun;74(3):375-84. doi: 10.1017/s0022172400046891.

Abstract

Inocula of polluted water naturally infected with salmonellas effectively distinguished six brands of selenite and six brands of tetrathionate enrichment media into satisfactory and unsatisfactory categories. Minimal inocula of pure cultures differentiated the tetrathionates, but not the selenites. Inocula of naturally infected chicken giblets suggested that there was a difference between two comparable brands of tetrathionate, but this was not statistically significant. The difference was, however, clearly demonstrated by minimal inocula of pure cultures. Intensive investigation of two inferior tetrathionates revealed inhomogeneity in the distribution of brilliant green in one bottle of one brand. The importance of the salmonella serotype and even the colonial variant used for the pure culture inoculum was also demonstrated.

摘要

自然感染沙门氏菌的污水接种物能有效地将六种品牌的亚硒酸盐和六种品牌的连四硫酸盐增菌培养基区分为满意和不满意两类。纯培养物的最小接种量能区分连四硫酸盐,但不能区分亚硒酸盐。自然感染鸡内脏的接种物表明,两种可比品牌的连四硫酸盐之间存在差异,但这在统计学上并不显著。然而,纯培养物的最小接种量清楚地证明了这种差异。对两种劣质连四硫酸盐的深入研究发现,一个品牌的一瓶培养基中亮绿的分布不均匀。还证明了沙门氏菌血清型甚至用于纯培养接种物的菌落变体的重要性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/108a/2130602/f6059bcd86da/jhyg00072-0084-a.jpg

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