Kidney B A, Ellis J A, Haines D M, Jackson M L
Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada.
Am J Vet Res. 2000 Sep;61(9):1037-41. doi: 10.2460/ajvr.2000.61.1037.
To evaluate the use of a polymerase chain reaction (PCR) method for detection of feline immunodeficiency virus (FIV) DNA, using formalin-fixed paraffin-embedded (FFPE) tissues, and to use this method to evaluate tissues obtained from vaccine site-associated sarcomas (VSS) of cats for FIV DNA.
50 FFPE tissue blocks from VSS of cats and 50 FFPE tissue blocks from cutaneous non-vaccine site-associated fibrosarcomas (non-VSS) of cats.
DNA was extracted from FFPE sections of each tumor and regions of the gag gene of FIV were amplified by a PCR, using 3 sets of primers. Sensitivity of the method was compared between frozen and FFPE tissues, using splenic tissue obtained from a cat that had been experimentally infected with FIV.
We did not detect FIV DNA in VSS or non-VSS tissues. Sensitivity of the PCR method was identical for frozen or FFPE tissues.
It is possible to detect FIV DNA in FFPE tissues by use of a PCR. We did not find evidence to support direct FIV involvement in the pathogenesis of VSS in cats.
评估使用聚合酶链反应（PCR）方法，利用福尔马林固定石蜡包埋（FFPE）组织检测猫免疫缺陷病毒（FIV）DNA，并使用该方法评估从猫的疫苗接种部位相关肉瘤（VSS）获取的组织中是否存在FIV DNA。
来自猫VSS的50个FFPE组织块和来自猫皮肤非疫苗接种部位相关纤维肉瘤（非VSS）的50个FFPE组织块。
从每个肿瘤的FFPE切片中提取DNA，并使用3组引物通过PCR扩增FIV的gag基因区域。使用从实验感染FIV的猫获得的脾脏组织，比较该方法在冷冻组织和FFPE组织之间的灵敏度。
我们在VSS或非VSS组织中未检测到FIV DNA。PCR方法在冷冻组织和FFPE组织中的灵敏度相同。
使用PCR可以在FFPE组织中检测到FIV DNA。我们没有找到证据支持FIV直接参与猫VSS的发病机制。
