Honda T, Finkelstein R A
Proc Natl Acad Sci U S A. 1979 Apr;76(4):2052-6. doi: 10.1073/pnas.76.4.2052.
After mutagenesis with nitrosoguanidine and selection by immuno-halo techniques, an avirulent mutant, designated Texas Star-SR, which produces no detectable A (active; ADP-ribosylating) region of the cholera enterotoxin (choleragen) but produces the B region (choleragenoid) in amounts similar to the hypertoxinogenic wild-type parent Vibrio cholerae (biotype E1 Tor serotype Ogawa), has been isolated. The mutant retains the colonizing ability, motility, prototrophy, and serologic characteristics of the parent. In relevant intestinal experimental models, it has been shown to be avirulent and to induce protection against challenge with virulent cholera vibrios. The mutant appears to be suitable for further evaluation in volunteers as a candidate living enteric vaccine against cholera and related enterotoxic enteropathies.
用亚硝基胍诱变并通过免疫晕圈技术筛选后，分离出一种无毒突变体，命名为Texas Star-SR，它不产生可检测到的霍乱肠毒素（霍乱原）的A（活性；ADP-核糖基化）区域，但产生的B区域（类霍乱原）的量与产毒力强的野生型亲本霍乱弧菌（E1 Tor生物型，小川血清型）相似。该突变体保留了亲本的定殖能力、运动性、原养型和血清学特征。在相关的肠道实验模型中，已证明它无毒，并能诱导对有毒霍乱弧菌攻击的保护作用。该突变体似乎适合作为抗霍乱及相关肠毒素性肠病的候选活肠溶疫苗在志愿者中进行进一步评估。
