Funada U, Wada M, Kawata T, Mori K, Tamai H, Kawanishi T, Kunou A, Tanaka N, Tadokoro T, Maekawa A
Faculty of Applied Bioscience, Tokyo University of Agriculture, Japan.
Int J Vitam Nutr Res. 2000 Jul;70(4):167-71. doi: 10.1024/0300-9831.70.4.167.
To clarify the role of vitamin B12 in the function of cell-mediated and humoral immune functions, the splenocytes expression of CD4, CD8 and serum C3, IgM, IgG concentrations were examined in vitamin B12-deficient rats, and the effect of the administration of methylcobalamin was also studied. The CD4+CD8-/CD4-CD8+ ratio in splenocytes was significantly higher in vitamin B12-deficient rats than in control rats (p < 0.05). The value in the 48 hours after methylcobalamin administration group, was within the normal range (p < 0.05). From these results, the elevation of the CD4+CD8-/CD4-CD8+ ratio by vitamin B12-deficiency was confirmed in rats. The serum C3, IgM and IgG concentrations were lower in the vitamin B12-deficient group than in the control group. These findings suggest that vitamin B12 plays a role in maintaining the immune function in rats.
为阐明维生素B12在细胞介导免疫功能和体液免疫功能中的作用，检测了维生素B12缺乏大鼠脾细胞中CD4、CD8的表达以及血清C3、IgM、IgG的浓度，并研究了甲钴胺给药的影响。维生素B12缺乏大鼠脾细胞中的CD4+CD8-/CD4-CD8+比值显著高于对照组大鼠（p<0.05）。甲钴胺给药组48小时后的该值在正常范围内（p<0.05）。从这些结果可以证实，维生素B12缺乏会使大鼠的CD4+CD8-/CD4-CD8+比值升高。维生素B12缺乏组的血清C3、IgM和IgG浓度低于对照组。这些发现表明维生素B12在维持大鼠免疫功能中发挥作用。
