Department of Pharmaceutical and Health Sciences, Faculty of Pharmacy, CEU San Pablo University, Boadilla del Monte, Madrid 28668, Spain.
Nutrients. 2013 Nov 26;5(12):4836-48. doi: 10.3390/nu5124836.
Different vitamin B12 and folic acid concentrations could exacerbate the immune response. The aim was to evaluate different dietary folic acid and vitamin B12 levels on the immune response in aged rats. Male Sprague Dawley aged rats were assigned to three folic acid groups (deficient, control, supplemented) each in absence of vitamin B12 for 30 days. Several parameters of innate and acquired immune responses were measured. Serum and hepatic folate levels increased according to folic acid dietary level, while vitamin B12 levels decreased. There was a significant decrease in natural killer cell-mediated cytotoxicity in the spleen for the vitamin B12 deficient diet and folic acid control diet groups. Significant changes in CD45 lymphocyte subsets were also observed according to dietary imbalance. Lymphoproliferative response to concanavalin A and phytohemagglutinin did not differ significantly between groups. The spleen response to lipopolysaccharide increased significantly, but was unmodified for the other organs. An imbalance between dietary vitamin B12 and folic acid concentrations alters some immunological parameters in aged rats. Therefore, the ratio between folate and vitamin B12 could be as important as their absolute dietary concentrations.
不同浓度的维生素 B12 和叶酸可能会加剧免疫反应。本研究旨在评估不同饮食中叶酸和维生素 B12 水平对老年大鼠免疫反应的影响。雄性 Sprague Dawley 老年大鼠被分为三组叶酸缺乏组、对照组和补充组，每组均在缺乏维生素 B12 的情况下喂养 30 天。检测了固有免疫和获得性免疫反应的多个参数。血清和肝叶酸水平随饮食中叶酸水平的增加而升高，而维生素 B12 水平则降低。维生素 B12 缺乏饮食组和叶酸对照组的脾自然杀伤细胞介导的细胞毒性显著降低。根据饮食失衡，还观察到 CD45 淋巴细胞亚群的显著变化。刀豆蛋白 A 和植物血凝素的淋巴细胞增殖反应在各组之间无显著差异。脂多糖对脾脏的反应显著增加，但对其他器官没有影响。饮食中维生素 B12 和叶酸浓度的不平衡会改变老年大鼠的一些免疫参数。因此，叶酸和维生素 B12 的比例可能与它们的绝对饮食浓度一样重要。
