Gangar V, Curiale M S, D'Onorio A, Schultz A, Johnson R L, Atrache V
Silliker Laboratories Group, Inc., Corporate Research Center, South Holland, IL 60473, USA.
J AOAC Int. 2000 Jul-Aug;83(4):903-18.
The VIDAS LIS method and the traditional culture methods for detection of Listeria species in food were evaluated in a multilaboratory comparative study. The 6 foods tested were either naturally contaminated or inoculated with 3 different concentrations of Listeria. Results for each food and each contamination level with the VIDAS LIS method were as good as or better than those obtained with the traditional culture method. Of 1558 samples tested, 935 were positive: 839 by the VIDAS method and 809 by standard culture methods. Overall false negative rates were 10.3 and 13.5% for the VIDAS LIS and culture methods, respectively. The false positive rate for the VIDAS LIS assay was 1.4% based on 9 VIDAS LIS positive assays that did not confirm positive by isolation of Listeria. The agreement between the VIDAS LIS and culture methods for all samples tested was 86%.
在一项多实验室比较研究中，对VIDAS LIS方法和传统培养方法检测食品中李斯特菌属的效果进行了评估。所检测的6种食品要么是自然污染的，要么接种了3种不同浓度的李斯特菌。VIDAS LIS方法对每种食品和每个污染水平的检测结果与传统培养方法相当或更好。在1558个检测样本中，935个呈阳性：VIDAS方法检测出839个阳性，标准培养方法检测出809个阳性。VIDAS LIS方法和培养方法的总体假阴性率分别为10.3%和13.5%。基于9次VIDAS LIS检测呈阳性但未通过李斯特菌分离确认阳性的结果，VIDAS LIS检测的假阳性率为1.4%。所检测的所有样本中，VIDAS LIS方法和培养方法之间的一致性为86%。
