Rh血型抗体的补体结合

Complement fixation by Rh blood group antibodies.

作者信息

Hidalgo C, Romano E L, Linares J, Suarez G

出版信息

Transfusion. 1979 May-Jun;19(3):250-4. doi: 10.1046/j.1537-2995.1979.19379204205.x.

DOI:10.1046/j.1537-2995.1979.19379204205.x

Abstract

Rh blood group antibodies normally do not fix complement. Rh positive intact red blood cells treated with papain do not lyse when incubated with corresponding antibody and complement. This study was done to determine if complement fixation occurs when antibodies were combined with Rh positive red blood cell ghosts untreated or treated with papain. Complement fixation was observed with IgG anti-D, anti-DC and anti-c when papain treated ghosts were used. No complement fixation or a smaller degree of it was observed in the case of untreated Rh positive red blood cell ghosts when incubated with similar anti-Rh antibodies. It is concluded that the papain treatment of Rh positive red blood cell ghosts, possibly by inducing aggregation of antigen sites, allowed complement binding by Rh antibodies.

摘要

Rh血型抗体通常不结合补体。用木瓜蛋白酶处理过的Rh阳性完整红细胞与相应抗体及补体一起孵育时不会溶解。本研究旨在确定抗体与未处理或经木瓜蛋白酶处理的Rh阳性红细胞膜空壳结合时是否会发生补体结合。当使用经木瓜蛋白酶处理的膜空壳时，观察到IgG抗-D、抗-DC和抗-c有补体结合现象。当未处理的Rh阳性红细胞膜空壳与相似的抗Rh抗体一起孵育时，未观察到补体结合或仅有较小程度的补体结合。得出的结论是，对Rh阳性红细胞膜空壳进行木瓜蛋白酶处理，可能是通过诱导抗原位点聚集，使得Rh抗体能够结合补体。