Marshall-Clarke S, Playfair J H
Immunology. 1975 Sep;29(3):477-86.
The affinity of anti-DNP antibodies produced by mice of various ages has been studied at the cellular level by the plaque inhibition technique. The affinity of PFC produced shortly after a single injection of antigen was found to increase during the first 6 weeks of life. The responses of 1- and 2-week-old animals also showed apparently restricted heterogeneity. The difference in the affinity of anti-DNP--PFC between young and adult mice could not be attributed to different (serum) levels of antigen or to differences in the rate of maturation of affinity during the immune response. Cell transfer experiments suggested that the age-dependent increase was due to a change in the population of antibody-forming (B) cell precursors and not to a progressive improvement of T-cell function. This findings is interpreted as favouring somatic mutation theories of antibody diversity.
通过蚀斑抑制技术在细胞水平上研究了不同年龄小鼠产生的抗二硝基苯酚(DNP)抗体的亲和力。发现单次注射抗原后不久产生的空斑形成细胞(PFC)的亲和力在出生后的前6周内增加。1周龄和2周龄动物的反应也表现出明显受限的异质性。幼年和成年小鼠之间抗DNP-PFC亲和力的差异不能归因于抗原的不同(血清)水平或免疫反应期间亲和力成熟速率的差异。细胞转移实验表明,年龄依赖性增加是由于抗体形成(B)细胞前体群体的变化,而不是T细胞功能的逐步改善。这一发现被解释为支持抗体多样性的体细胞突变理论。