Laforce F M, Mills D M, Iverson K, Cousins R, Everett E D
J Lab Clin Med. 1975 Oct;86(4):657-66.
The effect of normal serum and serum for seven patients with disseminated candidiasis (DC) on candidacidal activity of normal human neutrophils (PMNL) was studied. PMNL incubated in DC serum had depressed candiacidal activity when compared to studies with normal serum. The candidacidal defect was not related to differences in particle uptake since phagocytic indices were similar with either normal or DC serum. DC serum did not affect PMNL bactericidal activity against Staphylococcus aureus. When Candida albicans (CA) was incubated in either normal or DC sera, washed, and added to PMNL in normal serum, pronounced inhibition of candidacidal activity was seen in all studies with CA previously incubated in DC serum. Dilution of DC serum or absorption of DC serum with heat-killed CA reversed the candidacidal defect. Ultrastructural studies of phagocytized CA demonstrated small ("tight") phagolysosomes and a delay in breakdown of an electron-dense coating on CA previously incubated in DC serum when compared to controls. Inhibition of leukocyte candidacidal activity appeared to be related to high titers of IgG antibodies against CA present in DC serum.
研究了正常血清以及7例播散性念珠菌病（DC）患者的血清对正常人中性粒细胞（PMNL）杀念珠菌活性的影响。与用正常血清进行的研究相比，在DC血清中孵育的PMNL的杀念珠菌活性降低。杀念珠菌缺陷与颗粒摄取差异无关，因为正常血清或DC血清的吞噬指数相似。DC血清不影响PMNL对金黄色葡萄球菌的杀菌活性。当白色念珠菌（CA）在正常血清或DC血清中孵育、洗涤后，加入到正常血清中的PMNL中时，在所有用先前在DC血清中孵育的CA进行的研究中，均观察到杀念珠菌活性受到明显抑制。DC血清的稀释或用热灭活的CA对DC血清的吸收可逆转杀念珠菌缺陷。与对照组相比，对吞噬的CA进行超微结构研究显示，先前在DC血清中孵育的CA上有小的（“紧密的”）吞噬溶酶体，且电子致密包膜的分解延迟。白细胞杀念珠菌活性的抑制似乎与DC血清中存在的针对CA的高滴度IgG抗体有关。
