Desaire H, Leary J A
University of California, Berkeley 94720, USA.
J Am Soc Mass Spectrom. 2000 Oct;11(10):916-20. doi: 10.1016/S1044-0305(00)00168-9.
A new method of identifying and quantifying the disaccharide building blocks of glycosaminoglycans is introduced. The polysaccharides are subjected to an enzymatic digestion that releases the sulfated disaccharides. The disaccharides are then identified using a combination of electrospray ionization mass spectrometry and tandem mass spectrometry. Quantification of the isomeric disaccharides is also achieved by tandem mass spectrometry, using a recently developed methodology which quantifies mixtures of isomers without the use of chromatography or prior separation. Using mass spectrometry to characterize the components of glycosaminoglycans significantly reduces both sample consumption and analysis time of traditional methods.
本文介绍了一种鉴定和定量糖胺聚糖二糖组成单元的新方法。多糖经过酶解以释放硫酸化二糖。然后结合使用电喷雾电离质谱和串联质谱来鉴定二糖。还通过串联质谱，采用一种最近开发的无需色谱法或预先分离即可定量异构体混合物的方法，实现了异构体二糖的定量。使用质谱对糖胺聚糖的成分进行表征，显著减少了传统方法的样品消耗量和分析时间。
