Department of Chemistry, Indiana University , 800 East Kirkwood Avenue, Bloomington, Indiana 47405, United States.
Laboratoire de Chimie Physique Moléculaire, École Polytechnique Fédérale de Lausanne, EPFL SB ISIC LCPM , Station 6, CH-1015 Lausanne, Switzerland.
Anal Chem. 2017 Jul 18;89(14):7601-7606. doi: 10.1021/acs.analchem.7b01467. Epub 2017 Jul 7.
We combine ion mobility spectrometry with cryogenic, messenger-tagging, infrared spectroscopy and mass spectrometry to identify different isomeric disaccharides of chondroitin sulfate (CS) and heparan sulfate (HS), which are representatives of two major subclasses of glycosaminoglycans. Our analysis shows that while CS and HS disaccharide isomers have similar drift times, they can be uniquely distinguished by their vibrational spectrum between ∼3200 and 3700 cm due to their different OH hydrogen-bonding patterns. We suggest that this combination of techniques is well suited to identify and characterize glycan isomers directly, which presents tremendous challenges for existing methods.
我们将离子淌度谱与低温、信使标记、红外光谱和质谱相结合，以鉴定软骨素硫酸盐（CS）和硫酸乙酰肝素（HS）的不同异构二糖，它们分别是两种主要糖胺聚糖亚类的代表。我们的分析表明，虽然 CS 和 HS 二糖异构体具有相似的漂移时间，但由于它们不同的 OH 氢键模式，它们可以通过其在 ∼3200 至 3700cm 之间的振动光谱来独特地区分。我们建议这种技术组合非常适合直接识别和表征聚糖异构体，这对现有方法提出了巨大的挑战。
