Univ Lyon, Université Claude Bernard Lyon 1, CNRS, Institut Lumière Matière (ILM), F-69622, Villeurbanne, France.
Institut Universitaire de France IUF, 103 Blvd St Michel, 75005, Paris, France.
J Am Soc Mass Spectrom. 2018 Jun;29(6):1242-1249. doi: 10.1007/s13361-018-1955-5. Epub 2018 Apr 26.
We report distinctive spectroscopic fingerprints of the monosaccharide standards GalNAc4S and GalNAc6S by coupling mass spectrometry and ion spectroscopy in the 3-μm range. The disaccharide standards CSA and CSC are used to demonstrate the applicability of a novel approach for the analysis of sulfate position in GalNAc-containing glycosaminoglycans. This approach was then used for the analysis of a sample containing CSA and CSC disaccharides. Finally, we discuss the generalization of the coupling of mass spectrometry with ion spectroscopy for the structural analysis of glycosaminoglycans on a tetrasaccharide from dermatan sulfate source. Graphical abstract ᅟ.
我们通过在 3μm 范围内结合质谱和离子光谱，报告了单糖标准物 GalNAc4S 和 GalNAc6S 的独特光谱指纹。我们使用二糖标准物 CSA 和 CSC 来证明一种分析含 GalNAc 糖胺聚糖中硫酸酯位置的新方法的适用性。然后，我们使用该方法分析了含有 CSA 和 CSC 二糖的样品。最后，我们讨论了将质谱与离子光谱相结合用于分析源自硫酸皮肤素的四糖的糖胺聚糖结构的通用性。
