Iqbal M, Kenney P B, Al-Humadi N H, Klandorf H
Division of Animal and Veterinary Sciences, College of Agriculture, Forestry, and Consumer Sciences, West Virginia University, Morgantown 26506-6108, USA.
Poult Sci. 2000 Sep;79(9):1338-44. doi: 10.1093/ps/79.9.1338.
Nonenzymatic glycosylation contributes to the formation of crosslinks, which leads to the structural and functional deterioration of tissue protein. The accumulation of these crosslinks in tissue proteins has been implicated in the alteration of biomechanical properties of connective tissues. The objective of this study was to determine whether tendon breaking time (TBT) and tendon breaking strength (TBS) of the flexor perforans et perforatus digiti iii tendon were related to concentrations of pentosidine in tendons (Pt) of broiler breeder hens from 8 to 125 wk of age. In addition, effects of diet restriction (DR) and a crosslinking inhibitor, aminoguanidine (AG) on Pt, TBS, and TBT were determined. Female chicks (n = 450) were randomly assigned to four treatment groups immediately after hatch: ad libitum-fed (AL); diet-restricted (DR; 60% of AL); and AL and DR groups supplemented with 1.35 mg/kg BW per day AG in the feed (AL+AG and DR+AG, respectively). In AL hens, Pt increased with increasing age (P < or = 0.0001). Concurrently, an age-related parallel increase was found for TBS (P < or = 0.0001) and TBT (P < or = 0.0001). Rate of Pt accumulation was lower in DR (P < or = 0.001), TBS (P < or = 0.01), and TBT (P < or = 0.02) hens compared with AL hens. Concentration of Pt in the AL + AG group was lower (P < or = 0.0002) than in the AL group; TBS and TBT (P < or = 0.01) followed a similar pattern. Supplementation of DR with AG did not affect Pt, TBS, or TBT. The age-related increase in Pt and loss of elasticity in the tendon was retarded by diet restriction and AG.
非酶糖基化促进交联的形成，这会导致组织蛋白的结构和功能恶化。这些交联在组织蛋白中的积累与结缔组织生物力学特性的改变有关。本研究的目的是确定8至125周龄肉种鸡的屈趾深肌和屈趾浅肌腱的肌腱断裂时间（TBT）和肌腱断裂强度（TBS）是否与肌腱中戊糖苷（Pt）的浓度相关。此外，还测定了限饲（DR）和交联抑制剂氨基胍（AG）对Pt、TBS和TBT的影响。雌性雏鸡（n = 450）在孵化后立即随机分为四个处理组：自由采食（AL）；限饲（DR；AL的60%）；以及在饲料中每日添加1.35 mg/kg体重AG的AL组和DR组（分别为AL+AG和DR+AG）。在AL组母鸡中，Pt随年龄增长而增加（P≤0.0001）。同时，TBS（P≤0.0001）和TBT（P≤0.0001）也出现了与年龄相关的平行增加。与AL组母鸡相比，DR组母鸡的Pt积累速率较低（P≤0.001），TBS（P≤0.01）和TBT（P≤0.02）也是如此。AL + AG组的Pt浓度低于AL组（P≤0.0002）；TBS和TBT（P≤0.01）也呈现类似模式。在DR组中添加AG对Pt、TBS或TBT没有影响。限饲和AG减缓了Pt随年龄的增加以及肌腱弹性的丧失。
