Alarcón T, Domingo D, Prieto N, López-Brea M
Department of Microbiology, Hospital Universitario de la Princesa, Diego de León 62, 28006 Madrid, Spain.
J Antimicrob Chemother. 2000 Oct;46(4):613-6. doi: 10.1093/jac/46.4.613.
Thirty clarithromycin-resistant Helicobacter pylori strains (MIC range 8-64 mg/L) were subcultured in a drug-free medium and the MIC was determined every five passages to detect in vitro stability of resistance. Three out of the 30 (10%) lost their resistance after 10, 13 or 18 subcultures (MIC decrease from 8 to 0.008, from 16 to 0. 064 and from 32 to 0.016 mg/L). The effect of four macrolides at subinhibitory concentrations on the development of resistance was studied in H. pylori NCTC 11638 and TIGR 26695. A change in the MIC was observed only when NCTC11638 was exposed to 0.5 x MIC of erythromycin for 20 days.
30株对克拉霉素耐药的幽门螺杆菌菌株（最低抑菌浓度范围为8 - 64mg/L）在无药培养基中传代培养，每传5代测定一次最低抑菌浓度，以检测耐药性的体外稳定性。30株中有3株（10%）在传代10、13或18次后失去耐药性（最低抑菌浓度从8降至0.008mg/L，从16降至0.064mg/L，从32降至0.016mg/L）。研究了4种大环内酯类药物在亚抑菌浓度下对幽门螺杆菌NCTC 11638和TIGR 26695耐药性产生的影响。仅当NCTC11638暴露于0.5倍最低抑菌浓度的红霉素中20天时，才观察到最低抑菌浓度的变化。
