Kuwana T, Sato Y, Saka M, Kondo Y, Miyata M, Obara K, Nishimaki T, Kasukawa R
Second Department of Internal Medicine, Fukushima Medical University School of Medicine, Hikarigaoka, Japan.
J Gastroenterol. 2000;35(9):682-9. doi: 10.1007/s005350070047.
The presence of perinuclear anti-neutrophil cytoplasmic antibodies (P-ANCAs) and that of antibodies against cathepsin G, a target antigen for P-ANCAs, was determined in the sera of patients with ulcerative colitis (UC), relative to the endoscopic severity and disease activity. P-ANCAs were detected by indirect immunofluorescent assay (IIF) on ethanol-fixed human neutrophils. Antibodies to cathepsin G were detected by an enzyme-linked immunosorbent assay (ELISA) and Western blotting. P-ANCAs were detected by IIF in 62.5% of 32 patients with active UC. Anti-cathepsin G antibodies were detected in 40.6% of 32 patients with active UC, and their prevalence was significantly higher in patients with severe colitis, as determined by endoscopy, than in those with mild or moderate colitis (P < 0.05). The prevalence and titers of anti-cathepsin G antibodies were significantly higher during the active than the inactive phase of the disease (P < 0.05). Measurement of titers of anti-cathepsin G antibodies by ELISA in the serum is useful for evaluating the activity of UC.
相对于内镜严重程度和疾病活动度，测定了溃疡性结肠炎（UC）患者血清中核周抗中性粒细胞胞浆抗体（P-ANCAs）以及针对P-ANCAs靶抗原组织蛋白酶G的抗体的存在情况。通过对乙醇固定的人中性粒细胞进行间接免疫荧光测定（IIF）来检测P-ANCAs。通过酶联免疫吸附测定（ELISA）和蛋白质印迹法检测针对组织蛋白酶G的抗体。在32例活动期UC患者中，有62.5%通过IIF检测到P-ANCAs。在32例活动期UC患者中，有40.6%检测到抗组织蛋白酶G抗体，并且通过内镜检查确定，重度结肠炎患者中该抗体的患病率显著高于轻度或中度结肠炎患者（P<0.05）。疾病活动期抗组织蛋白酶G抗体的患病率和滴度显著高于非活动期（P<0.05）。通过ELISA测定血清中抗组织蛋白酶G抗体的滴度有助于评估UC的活动度。
