Koukila-Kähkölä Pirkko, Paulin Lars, Brander Eljas, Jantzen Erik, Eho-Remes Mirja, Katila Marja-Leena
*Institute of Biotechnology, University of Helsinki, 00014 Helsinki, †Helsinki University Central Hospital, 00250 Helsinki, ‡United Laboratories Ltd, 00381 Helsinki, Finland, §Department of Vaccine, National Institute of Public Health, 0461 Oslo, Norway, ||Peijas Hospital, 01400 Vantaa and **Clinical Microbiology, Kuopio University Hospital, 70211 Kuopio, Finland.
J Med Microbiol. 2000 Oct;49(10):937-940. doi: 10.1099/0022-1317-49-10-937.
This report describes the first isolation of Mycobacterium shimoidei in Finland from a sputum specimen obtained from an elderly female patient. M. shimoidei, a potential lung pathogen, is difficult to identify by routine methods and only a few cases have been reported. The present study demonstrated that M. shimoidei has a characteristic pattern for fatty acids and alcohols in gas liquid chromatography. This chromatogram and the pattern of mycolic acids on thin-layer chromatography allow it to be distinguished routinely. The unique sequence of the 16S rRNA gene and the 16S-23S rDNA spacer region allows identification by molecular methods.
本报告描述了在芬兰首次从一名老年女性患者的痰液标本中分离出下田分枝杆菌。下田分枝杆菌是一种潜在的肺部病原体，难以通过常规方法鉴定，仅有少数病例报道。本研究表明，下田分枝杆菌在气相色谱法中具有脂肪酸和醇类的特征图谱。该色谱图以及薄层色谱法中分枝菌酸的图谱使其能够常规鉴别。16S rRNA基因和16S - 23S rDNA间隔区的独特序列使得能够通过分子方法进行鉴定。
