González-Rey C, Svenson S B, Bravo L, Rosinsky J, Ciznar I, Krovacek K
Department of Veterinary Microbiology, SLU, Biomedical Center, Uppsala, Sweden.
FEMS Immunol Med Microbiol. 2000 Oct;29(2):107-13. doi: 10.1111/j.1574-695X.2000.tb01512.x.
Twenty-five strains of Plesiomonas shigelloides isolated from aquatic environment, 10 strains from human cases of diarrhoea and five strains from animals were identified by the polymerase chain reaction technique based on 23S rRNA gene. For this purpose, two primers targeted against part of the 5' half of the 23S rRNA gene of P. shigelloides (Escherichia coli number C-912, G-1195; Plesiomonas number C-906, G-1189) were designed. Results from our study indicated that this method might serve as a tool for a rapid and sensitive identification of P. shigelloides from different environmental and clinical sources.
利用基于23S rRNA基因的聚合酶链反应技术，对从水生环境中分离出的25株类志贺邻单胞菌、10株人类腹泻病例菌株以及5株动物源菌株进行了鉴定。为此，设计了针对类志贺邻单胞菌23S rRNA基因5' 端一半区域的两条引物（大肠杆菌编号C - 912、G - 1195；类志贺邻单胞菌编号C - 906、G - 1189）。我们的研究结果表明，该方法可作为一种从不同环境和临床来源快速、灵敏地鉴定类志贺邻单胞菌的工具。
