Ogle C K, Kong F, Guo X, Wells D A, Aosasa S, Noel G, Horseman N
Shriners Hospital for Children, Cincinnati, Ohio 45229, USA.
Shock. 2000 Sep;14(3):392-8; discussion 398-9.
The newly identified suppressors of cytokine signaling (SOCS) family of proteins act as intracellular inhibitors of several cytokine signal transduction pathways. Their expression is induced by cytokine activation of the Janus kinase/signal transducer and activator of transcription (Jak/STAT) pathway, and they act as a negative feedback loop by subsequently inhibiting the Jak/STAT pathway either by direct interaction with activated Jaks or with the receptors. In this study we investigated the expression and translation of SOCS proteins after burn injury. Thermal injury increased the expression of SOCS3 compared with sham at 4 h, 24 h, and 10 days after thermal injury in the liver. SOCS3 protein was increased at 4 and 24 h after thermal injury in the liver. Expression of SOCS1 mRNA was not detected in sham or burn liver. SOCS2 mRNA and cytokine-inducible SH2-containing protein (CIS) mRNA were detected at the same levels for both sham and burn at all time points in the liver. In the spleen there was a trend towards an increase in SOCS1 mRNA at all time points; thermal injury significantly decreased SOCS2 mRNA compared with sham at 4 h, SOCS3 mRNA was significantly increased at 24 h compared with 10 days, and CIS mRNA was detected at the same levels for both sham and burn at all time points. In conclusion, thermal injury causes elevations in SOCS3 within 4 h after a burn, reaching a maximum at 24 h post injury. Levels continue to be elevated for up to 10 days post injury. SOCS3 may be very important in regulating the balance between immunosuppression and inflammation after thermal injury.
新发现的细胞因子信号转导抑制因子（SOCS）蛋白家族作为几种细胞因子信号转导途径的细胞内抑制剂发挥作用。它们的表达由Janus激酶/信号转导子和转录激活子（Jak/STAT）途径的细胞因子激活诱导，随后它们通过直接与活化的Jaks或受体相互作用来抑制Jak/STAT途径，从而起到负反馈环的作用。在本研究中，我们调查了烧伤后SOCS蛋白的表达和翻译情况。与假手术组相比，热损伤后4小时、24小时和10天时，肝脏中SOCS3的表达增加。热损伤后4小时和24小时，肝脏中SOCS3蛋白增加。在假手术组或烧伤肝脏中均未检测到SOCS1 mRNA的表达。在肝脏的所有时间点，假手术组和烧伤组中SOCS2 mRNA和细胞因子诱导含SH2蛋白（CIS）mRNA的检测水平相同。在脾脏中，所有时间点SOCS1 mRNA均有增加的趋势；与假手术组相比热损伤后4小时SOCS2 mRNA显著降低，与10天时相比热损伤后24小时SOCS3 mRNA显著增加，在所有时间点假手术组和烧伤组中CIS mRNA的检测水平相同。总之，热损伤导致烧伤后4小时内SOCS3升高，在损伤后24小时达到最大值。损伤后长达10天其水平持续升高。SOCS3在调节热损伤后免疫抑制和炎症之间的平衡中可能非常重要。
