Tung K S, Hong E J, Roeder G S
Howard Hughes Medical Institute, Yale University, New Haven, CT 06520-8103, USA.
Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):12187-92. doi: 10.1073/pnas.220464597.
In budding yeast, many mutants defective in meiotic recombination and chromosome synapsis undergo checkpoint-mediated arrest at the pachytene stage of meiotic prophase. We recovered the NDT80 gene in a screen for genes whose overexpression bypasses the pachytene checkpoint. Ndt80 is a meiosis-specific transcription factor that promotes expression of genes required for exit from pachytene and entry into meiosis I. Herein, we show that the Ndt80 protein accumulates and is extensively phosphorylated during meiosis in wild type but not in cells arrested at the pachytene checkpoint. Our results indicate that inhibition of Ndt80 activity is one mechanism used to achieve pachytene arrest.
在出芽酵母中，许多减数分裂重组和染色体联会存在缺陷的突变体在减数分裂前期的粗线期会经历检查点介导的细胞停滞。我们在一项筛选中发现了NDT80基因，该筛选旨在寻找那些过表达可绕过粗线期检查点的基因。Ndt80是一种减数分裂特异性转录因子，可促进粗线期退出及进入减数分裂I所需基因的表达。在此，我们表明，在野生型减数分裂过程中Ndt80蛋白会积累并大量磷酸化，但在粗线期检查点停滞的细胞中则不会。我们的结果表明，抑制Ndt80活性是实现粗线期停滞的一种机制。
