Goto K, Sugiyama K, Ando T, Mizutani F, Terabe K, Tanaka K, Nishiyama M, Wada Y
Department of Pediatrics Nagoya City University Medical School, Nagoya, Japan.
Tohoku J Exp Med. 2000 Aug;191(4):203-7. doi: 10.1620/tjem.191.203.
To date, the routes of mother-to-infant transmission of TT virus (TTV) have not been fully elucidated. The present study examines the detection rates of TTV DNA in the serum of pregnant Japanese women and in cord blood at the time of delivery, as well as in the saliva and breast milk of mothers one-month postpartum. Primers derived from the well-known translated region N22 (N22 system), as well as the untranslated region (UTR system) were used. The prevalence of TTV DNA in the serum of pregnant women was found to be 11.9% (19/160) using the N22 system and 72.4% (55/76) using the UTR system. No TTV DNA was detected in the cord blood samples (0/160) when the N22 system was used for detection but TTV DNA was detected in 11.8% (7/76) of samples studied with the UTR system. Using the N22 system, TTV DNA was not detected in breast milk, but was detected in saliva. However using the UTR system, TTV DNA was detected in both specimens. These results imply that some babies are vertically infected with TTV via cord blood at the time of delivery or via breast milk or saliva. However, further research is necessary to confirm this hypothesis. polymerase chain reaction; pregnant women; horizontal route of transmission
迄今为止，TT病毒（TTV）母婴传播的途径尚未完全阐明。本研究检测了日本孕妇血清、分娩时脐带血以及产后1个月母亲唾液和母乳中TTV DNA的检出率。使用了源自著名的编码区N22（N22系统）以及非编码区（UTR系统）的引物。使用N22系统时，孕妇血清中TTV DNA的流行率为11.9%（19/160），使用UTR系统时为72.4%（55/76）。当使用N22系统检测时，脐带血样本中未检测到TTV DNA（0/160），但使用UTR系统检测的样本中有11.8%（7/76）检测到TTV DNA。使用N22系统时，母乳中未检测到TTV DNA，但在唾液中检测到。然而，使用UTR系统时，在这两种样本中均检测到TTV DNA。这些结果表明，一些婴儿在分娩时通过脐带血或通过母乳或唾液发生TTV垂直感染。然而，需要进一步研究来证实这一假设。聚合酶链反应；孕妇；水平传播途径
