Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Oparina 4, Moscow, 117513, Russia.
Pirogov Russian National Research Medical University, Ostrovityanova 1, Moscow, 117997, Russia.
Virol J. 2018 May 30;15(1):96. doi: 10.1186/s12985-018-1007-6.
Torque teno virus is a small chronically persisting circular negative ssDNA virus reaching near 100% prevalence. It is reported to be a marker for immune function in immunocompromised patients. The possibility of vertical maternal-fetal transmission remains controversial but incidence rate of TTV DNA in children increased with age. TTV dynamics well studied for allogeneic hematopoietic stem cell transplantation as a predictor of post-transplant complications but there is no viral proliferation kinetics data for other patient groups or healthy individuals. The aim of this study was to determine TTV dynamics during the first year of life of healthy infants.
Ninety eight clinically healthy breastfeeding infants (1-12 months of age) were analyzed by quantitative PCR for the whole blood TTV load with the test sensitivity of about 1000 viral copies per milliliter of blood (total number of samples including repeatedly tested infants was 109).
67% of all analyzed samples were TTV-positive demonstrating significant positive correlation between age and TTV load (r = 0.81, p < 0.01).
This is the first study to suggest that viral load increases during the first year of life reaching a plateau after 6 months with strong proliferation for the first 60 days. Our data well correlates with TTV dynamics in patients following allogeneic hematopoietic stem cell transplantation.
Torque teno 病毒是一种小型慢性持续的环状负 ssDNA 病毒，流行率接近 100%。据报道，它是免疫功能低下患者的免疫标志物。垂直母婴传播的可能性仍存在争议，但 TTV DNA 在儿童中的发生率随年龄增长而增加。TTV 在异基因造血干细胞移植中作为移植后并发症的预测指标已得到充分研究，但对于其他患者群体或健康个体，尚无病毒增殖动力学数据。本研究旨在确定健康婴儿生命第一年 TTV 的动态变化。
通过定量 PCR 分析 98 例临床健康母乳喂养婴儿（1-12 个月龄）的全血 TTV 负荷，检测灵敏度约为每毫升血液 1000 个病毒拷贝（包括重复测试婴儿的总样本数为 109）。
所有分析样本中有 67%为 TTV 阳性，表明年龄与 TTV 负荷之间存在显著正相关（r=0.81，p<0.01）。
这是第一项表明病毒载量在生命的第一年增加并在 6 个月后达到平台期的研究，在前 60 天具有强烈增殖的研究。我们的数据与异基因造血干细胞移植后患者的 TTV 动态变化密切相关。
