Hydén H, Rönnbäck L
Brain Res. 1975 Dec 26;100(3):615-28. doi: 10.1016/0006-8993(75)90161-4.
In this study we present data supporting a heterogeneous distribution of the brain-specific S-100 protein on nerve cell plasma membranes from isolated neurons. This has been shown in a previous study by immunoelectron microscopy using peroxidase-conjugated anti-S-100 antiserum and in this study by immunofluorescence using fluorescein-conjugated anti-S-100 antiserum, the specificity of which is discussed. Incubation of the cells at +23 degrees C or at +4 degrees C did not change the distribution pattern of S-100 over the cell membranes, nor did incubation in antimetabolites, or incubation with univalent Fab fragments. We conclude that the membrane-bound S-100 protein with its heterogeneous and polar distribution on the nerve cells is a sign of a protein differentiation of the neuron.
在本研究中，我们提供的数据支持脑特异性S-100蛋白在分离神经元的神经细胞质膜上呈异质性分布。先前的一项研究通过使用过氧化物酶偶联抗S-100抗血清的免疫电子显微镜已证明了这一点，而在本研究中则通过使用荧光素偶联抗S-100抗血清的免疫荧光法进行了证明，文中还讨论了其特异性。将细胞在23℃或4℃下孵育不会改变S-100在细胞膜上的分布模式，在抗代谢物中孵育或与单价Fab片段孵育也不会改变。我们得出结论，膜结合的S-100蛋白在神经细胞上具有异质性和极性分布，这是神经元蛋白质分化的一个标志。
