Hydén H, Rönnbäck L
J Neurol Sci. 1978 Dec;39(2-3):157-67. doi: 10.1016/0022-510x(78)90120-x.
The distribution of nervous tissue specific S-100 and 14.3.2 proteins was studied over the total surface of isolated, intact nerve cells by immunofluorescence microscopy. The distribution of the antigens was different on the front and back of the cells, indicating complicated S-100 and 14.3.2 patterns. A partly overlapping in membrane pattern of S-100 and 14.3.2 proteins was observed, although S-100 protein dominated with respect to membrane areas covered. At higher resolution the specific fluorescence appeared as conglomerates and islets. The neuronal membrane patterns of S-100 and 14.3.2 antigens, including synapses suggest that nerve cell membranes are functionally differentiated, thus greatly increasing the capacity for identification of incoming stimuli.
通过免疫荧光显微镜研究了神经组织特异性S-100和14.3.2蛋白在分离的完整神经细胞整个表面的分布情况。抗原在细胞的正面和背面分布不同，表明S-100和14.3.2蛋白呈现复杂的分布模式。尽管在覆盖的膜面积方面S-100蛋白占主导，但观察到S-100和14.3.2蛋白的膜分布模式有部分重叠。在更高分辨率下，特异性荧光呈现为聚集体和小岛状。S-100和14.3.2抗原的神经元膜分布模式，包括突触，表明神经细胞膜在功能上是分化的，从而大大提高了识别传入刺激的能力。
