Distribution of S-100 and 14-3-2 proteins on neuronal cell membranes.

The distribution of nervous tissue specific S-100 and 14.3.2 proteins was studied over the total surface of isolated, intact nerve cells by immunofluorescence microscopy. The distribution of the antigens was different on the front and back of the cells, indicating complicated S-100 and 14.3.2 patterns. A partly overlapping in membrane pattern of S-100 and 14.3.2 proteins was observed, although S-100 protein dominated with respect to membrane areas covered. At higher resolution the specific fluorescence appeared as conglomerates and islets. The neuronal membrane patterns of S-100 and 14.3.2 antigens, including synapses suggest that nerve cell membranes are functionally differentiated, thus greatly increasing the capacity for identification of incoming stimuli.