Hydén H
Proc Natl Acad Sci U S A. 1974 Aug;71(8):2965-8. doi: 10.1073/pnas.71.8.2965.
A theory is presented according to which the neuronal membrane and synapses are influenced by membrane-bound S100 protein and a membrane-associated network of actin-like, 20-A-diameter, coiled filaments and the competition by both proteins for Ca(2+). The discussion is based on observations made with fluorescein- and peroxidase-conjugated antiserum against S100 protein on isolated nerve cells. A small part of the S100 protein is membrane-bound in a polar localization that develops postnatally. The competition for Ca(2+) can influence the state of the network filaments which uncoil on binding Ca(2+) and affect differently the conformational state of synapses and membrane depending on the S100 protein content.
提出了一种理论，根据该理论，神经元膜和突触受膜结合的S100蛋白、直径20埃的肌动蛋白样卷曲细丝组成的膜相关网络以及这两种蛋白对Ca(2+)的竞争影响。讨论基于用针对S100蛋白的荧光素和过氧化物酶偶联抗血清对分离的神经细胞进行的观察。一小部分S100蛋白以极性定位的方式结合在膜上，这种定位在出生后形成。对Ca(2+)的竞争可影响网络细丝的状态，细丝在结合Ca(2+)时会展开，并根据S100蛋白的含量对突触和膜的构象状态产生不同影响。
