电刺激对胞浆内单精子注射过程中牛卵母细胞激活和胚胎发育的影响。
Effects of electric stimulation on bovine oocyte activation and embryo development in intracytoplasmic sperm injection procedure.
作者信息
Hwang S, Lee E, Yoon J, Yoon B K, Lee J H, Choi D
机构信息
Infertility Clinic, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
出版信息
J Assist Reprod Genet. 2000 Jul;17(6):310-4. doi: 10.1023/a:1009496726343.
PURPOSE
This study was carried out to investigate the efficacy of electric stimulation before and/or after intracytoplasmic sperm injection (ICSI) on bovine oocyte activation and embryo development.
METHODS
The oocytes were treated with electric shock before (B), before and after (B&A), and after (A) sperm injection. In each group, sham ICSI (ICSI-s) was performed to exclude the effect of parthenogenesis (B ICSI-s, B&A ICSI-s, and A ICSI-s). An electric pulse was applied with a single direct current (DC) pulse (0.8 kV/cm, 70 microseconds).
RESULTS
One pronucleus (PN) formation in the B&A ICSI-s group was slightly higher than that found in B and B&A ICSI group; however, the difference was not significant. Two PN formation in B&A ICSI group was higher than that found in sham ICSI groups (P < 0.05). There were no differences among treatment groups in the cleavage rate; however, morulae and blastocyst formation in the B&A embryos was significantly higher than that of other groups (P < 0.05) and got pregnant.
CONCLUSIONS
Electric stimulation before and after injection was an effective method in inducing bovine oocyte activation and in sustaining embryo development to the morulae and blastocyst stage.
目的
本研究旨在探讨胞浆内单精子注射（ICSI）前后电刺激对牛卵母细胞激活和胚胎发育的效果。
方法
卵母细胞在精子注射前（B组）、注射前后（B&A组）以及注射后（A组）接受电休克处理。每组均进行假ICSI（ICSI-s）以排除孤雌生殖的影响（B ICSI-s组、B&A ICSI-s组和A ICSI-s组）。施加单个直流（DC）脉冲（0.8 kV/cm，70微秒）的电脉冲。
结果
B&A ICSI-s组单原核（PN）形成略高于B组和B&A ICSI组，但差异不显著。B&A ICSI组双PN形成高于假ICSI组（P<0.05）。各处理组间的卵裂率无差异；然而，B&A胚胎的桑椹胚和囊胚形成显著高于其他组（P<0.05）并成功妊娠。
结论
注射前后的电刺激是诱导牛卵母细胞激活并维持胚胎发育至桑椹胚和囊胚阶段的有效方法。
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