短暂配子共孵育对人类体外受精的影响。
The effects of brief gamete co-incubation in human in vitro fertilization.
作者信息
Lin S P, Lee R K, Su J T, Lin M H, Hwu Y M
机构信息
Department of Medical Research, Mackay Memorial Hospital, Tamshui, Taipei, Taiwan.
出版信息
J Assist Reprod Genet. 2000 Jul;17(6):344-8. doi: 10.1023/a:1009409229068.
Abstract
PURPOSE
To investigate whether very short exposure of mature oocytes to sperm in vitro may affect the fertilization rates, embryo cleavage rates, and embryo quality between sibling oocytes in the same patient.
METHODS
Sibling oocytes of the same patient from 23 oocyte collection cycles were randomly allocated to the study group, with a 1-hr or 3-hr sperm-oocyte incubation, or the control group with the standard overnight gamete co-incubation. The fertilization rates, cleavage rates, and subsequent embryo quality were evaluated.
RESULTS
Our results showed no statistically significant differences in fertilization rates, embryo cleavage rates, and quality of the embryos between the study group and the control group.
CONCLUSIONS
Since the present study showed that long exposure of the oocyte to sperm has no advantage over short exposure, we prefer shortening the oocyte-sperm incubation period for reducing the negative effect induced by nonphysiologically high concentrations of spermatozoa.
摘要
目的
研究成熟卵母细胞在体外与精子的极短时间接触是否会影响同一患者同胞卵母细胞之间的受精率、胚胎分裂率和胚胎质量。
方法
从23个卵母细胞采集周期中选取同一患者的同胞卵母细胞，随机分为研究组，进行1小时或3小时的精卵共孵育，以及对照组，进行标准的过夜配子共孵育。评估受精率、分裂率和随后的胚胎质量。
结果
我们的结果显示，研究组和对照组之间在受精率、胚胎分裂率和胚胎质量方面没有统计学上的显著差异。
结论
由于本研究表明卵母细胞与精子的长时间接触并不比短时间接触更具优势，我们倾向于缩短精卵孵育时间，以减少非生理性高浓度精子诱导的负面影响。
相似文献
1
The effects of brief gamete co-incubation in human in vitro fertilization.短暂配子共孵育对人类体外受精的影响。
J Assist Reprod Genet. 2000 Jul;17(6):344-8. doi: 10.1023/a:1009409229068.
2
The effect of gamete co-incubation time during in vitro fertilization with frozen-thawed unsorted and sex-sorted ram spermatozoa on the development of in vitro matured adult and prepubertal ewe oocytes.体外受精时，冷冻解冻的未分选和按性别分选的公羊精子的配子共孵育时间对体外成熟的成年和青春期前母羊卵母细胞发育的影响。
Theriogenology. 2005 Jul 15;64(2):363-77. doi: 10.1016/j.theriogenology.2004.12.004.
3
Effects of modification of in vitro fertilization techniques on the sex ratio of the resultant bovine embryos.体外受精技术改良对所得牛胚胎性别比例的影响。
Anim Reprod Sci. 2008 May;105(3-4):234-44. doi: 10.1016/j.anireprosci.2007.03.006. Epub 2007 Mar 7.
4
Effect of co-incubation of oocytes with a decreasing number of spermatozoa on embryo quality.卵母细胞与逐渐减少的精子共同孵育对胚胎质量的影响。
Reprod Biomed Online. 2013 Apr;26(4):353-9. doi: 10.1016/j.rbmo.2012.12.009. Epub 2012 Dec 28.
5
Adjustments in IVF system for individual boars: value of additives and time of sperm-oocyte co-incubation.针对个体公猪的体外受精（IVF）系统调整：添加剂的作用及精卵共孵育时间
Theriogenology. 2005 Nov;64(8):1783-96. doi: 10.1016/j.theriogenology.2005.04.008.
6
Effects of reducing insemination time in human in vitro fertilization and embryo development by using sibling oocytes.利用同胞卵母细胞缩短人类体外受精授精时间对胚胎发育的影响
J Assist Reprod Genet. 1998 Nov;15(10):605-8. doi: 10.1023/a:1020333427698.
7
A prospective study, using sibling oocytes, examining the effect of 30 seconds versus 90 minutes gamete co-incubation in IVF.一项前瞻性研究，使用同胞卵母细胞，探讨在体外受精中配子共孵育30秒与90分钟的效果。
Hum Reprod. 2006 Feb;21(2):518-23. doi: 10.1093/humrep/dei350. Epub 2005 Oct 20.
8
Sperm binding, in vitro fertilization, and in vitro embryonic development of bovine oocytes fertilized with spermatozoa incubated with norepinephrine.用去甲肾上腺素孵育的精子使牛卵母细胞受精后的精子结合、体外受精及体外胚胎发育
Anim Reprod Sci. 2006 Nov;96(1-2):1-9. doi: 10.1016/j.anireprosci.2005.10.006. Epub 2005 Nov 21.
9
Brief coincubation of gametes in porcine in vitro fertilization: role of sperm:oocyte ratio and post-coincubation medium.猪体外受精中配子的短暂共孵育：精子与卵子比例及共孵育后培养基的作用
Theriogenology. 2007 Feb;67(3):620-6. doi: 10.1016/j.theriogenology.2006.09.022. Epub 2006 Oct 19.
10
A randomized study comparing the effect of standard versus short incubation of sperm and oocyte on subsequent pregnancy and implantation rates following in vitro fertilization embryo transfer.
Arch Androl. 2000 Jul-Aug;45(1):73-6. doi: 10.1080/014850100410042.
引用本文的文献
1
The clinical value of short-term insemination: a retrospective analysis of 3496 patients.短期授精的临床价值：对3496例患者的回顾性分析
BMC Pregnancy Childbirth. 2025 Jan 14;25(1):30. doi: 10.1186/s12884-025-07151-9.
2
Brief and long co-incubation of sperm and oocytes for in vitro fertilization: a meta-analysis of randomized controlled trials.短时间和长时间共孵育精子和卵子进行体外受精：一项随机对照试验的荟萃分析。
BMC Pregnancy Childbirth. 2023 Mar 23;23(1):200. doi: 10.1186/s12884-023-05490-z.
3
Opportunities and Limits of Conventional IVF versus ICSI: It Is Time to Come off the Fence.常规体外受精与卵胞浆内单精子注射的机遇与局限：是时候表明立场了。
J Clin Med. 2022 Sep 27;11(19):5722. doi: 10.3390/jcm11195722.
4
Long-time vs. short-time insemination of sibling eggs.同胞卵的长时间与短时间授精
Exp Ther Med. 2016 Dec;12(6):3756-3760. doi: 10.3892/etm.2016.3827. Epub 2016 Oct 20.
5
Does reducing gamete co-incubation time improve clinical outcomes: a retrospective study.缩短配子共同孵育时间是否能改善临床结局：一项回顾性研究
J Assist Reprod Genet. 2016 Jan;33(1):33-8. doi: 10.1007/s10815-015-0618-8. Epub 2015 Dec 2.
6
Brief co-incubation of sperm and oocytes for in vitro fertilization techniques.用于体外受精技术的精子与卵母细胞的短暂共同孵育。
Cochrane Database Syst Rev. 2013 Apr 30;2013(4):CD009391. doi: 10.1002/14651858.CD009391.pub2.
7
Short gamete co-incubation during in vitro fertilization decreases the fertilization rate and does not improve embryo quality: a prospective auto controlled study.体外受精期间短时间配子共孵育会降低受精率且不会改善胚胎质量：一项前瞻性自身对照研究。
J Assist Reprod Genet. 2008 Jul;25(7):305-10. doi: 10.1007/s10815-008-9240-3. Epub 2008 Aug 1.
本文引用的文献
1
Oocyte quality and treatment outcome in intracytoplasmic sperm injection cycles of polycystic ovarian syndrome patients.
Hum Reprod. 1999 Feb;14(2):354-8. doi: 10.1093/humrep/14.2.354.
2
A successful pregnancy with in vitro fertilization and embryo transfer in an infertile woman with Kartagener's syndrome: a case report.一名患有卡塔格内综合征的不孕女性通过体外受精和胚胎移植成功受孕：病例报告
J Assist Reprod Genet. 1998 Nov;15(10):625-7. doi: 10.1023/a:1020341629516.
3
Confirmation of the beneficial effects of brief coincubation of gametes in human in vitro fertilization.
Fertil Steril. 1998 Mar;69(3):399-402. doi: 10.1016/s0015-0282(97)00576-1.
4
Prolonged sperm-oocyte exposure and high sperm concentration affect human embryo viability and pregnancy rate.精子与卵母细胞长时间接触以及高精子浓度会影响人类胚胎活力和妊娠率。
Hum Reprod. 1996 Nov;11(11):2507-11. doi: 10.1093/oxfordjournals.humrep.a019149.
5
Reducing the time of sperm-oocyte interaction in human in-vitro fertilization improves the implantation rate.在人类体外受精过程中缩短精卵相互作用时间可提高着床率。
Hum Reprod. 1996 Jan;11(1):166-71. doi: 10.1093/oxfordjournals.humrep.a019011.
6
Influence of sperm parameters on embryo quality.精子参数对胚胎质量的影响。
Fertil Steril. 1993 Nov;60(5):888-92. doi: 10.1016/s0015-0282(16)56292-x.
7
Pathophysiology of human spermatozoa.
Curr Opin Obstet Gynecol. 1994 Apr;6(2):128-35.
8
A free radical theory of male infertility.男性不育的自由基理论。
Reprod Fertil Dev. 1994;6(1):19-23; discussion 23-4. doi: 10.1071/rd9940019.
9
Superoxide anion in human semen related to seminal parameters and clinical aspects.人类精液中的超氧阴离子与精液参数及临床情况的关系。
Fertil Steril. 1994 Oct;62(4):862-8. doi: 10.1016/s0015-0282(16)57017-4.
10
Sperm morphologic features as a prognostic factor in in vitro fertilization.精子形态特征作为体外受精的一个预后因素。
Fertil Steril. 1986 Dec;46(6):1118-23. doi: 10.1016/s0015-0282(16)49891-2.
Zotero 插件