Ulevitch R J, Cochrane C G, Henson P M, Morrison D C, Doe W F
J Exp Med. 1975 Dec 1;142(6):1570-90. doi: 10.1084/jem.142.6.1570.
We have studied the role of complement in lipopolysaccharide (LPS)-induced hypotension and disseminated intravascular coagulation (DIC) by comparing the effects of injection of three preparations of LPS from E. Coli 0111:B4, S. minnesota Re595, and S. marcescens. Injections of nonlethal doses of these LPS preparations into normal rabbits produced decreases in mean arterial blood pressure during a 5-h period. When rabbits treated with cobra venom factor (CoF) to deplete C3 were injected with the various LPS preparations, mean arterial pressures fell at a rate and extent essentially identical to that observed in normal rabbits. Rabbits genetically deficient in C6 also demonstrated LPS-induced hypotensive changes. Only minimal, or no changes in plasma C3 levels or serum CH50 values were detected in normal rabbits after LPS injection. Hypotensive changes were also induced in rabbits when complement was rapidly activated by intravenous injection of CoF. In contrast to the hypotension induced by LPS, the fall in arterial pressure associated with the consumption of complement was short lived and required the rapid consumption of considerable amounts of C3. The occurrence of DIC noted in normal rabbits injected with each preparation of LPS was not inhibited in either rabbits treated with cobra factor or in C6-deficient rabbits. The DIC was most pronounced after injection of Re595 and S. marcescens LPS. Injection of the various LPS preparations produced a rapid disappearance of circulating neutrophils and mononuclear cells, which occurred with the same kinetics and to the same extent in normal, CoF-treated, and C6-deficient rabbits. Injection of either Re595 LPS or S. marcescens LPS produced a biphasic disappearance of circulating 51Cr-platelets. In contrast, injection of 0111:B4 LPS affected only slightly the rate of disappearance of 51Cr-platelets. Depletion of C3 by cobra factor treatment had no effect on the disappearance of platelets in animals injected with 0111:B4. In marked contrast cobra factor treatment greatly reduced the initial rapid disappearance of platelets in rabbits injected with either Re595 or S. marcescens LPS, but had no effect in the secondary disappearance phase.
我们通过比较注射三种来自大肠杆菌0111:B4、明尼苏达沙门氏菌Re595和粘质沙雷氏菌的脂多糖（LPS）制剂的效果，研究了补体在LPS诱导的低血压和弥散性血管内凝血（DIC）中的作用。将这些LPS制剂的非致死剂量注射到正常兔体内，在5小时内平均动脉血压下降。当用眼镜蛇毒因子（CoF）处理以耗尽C3的兔注射各种LPS制剂时，平均动脉压下降的速率和程度与正常兔中观察到的基本相同。C6基因缺陷的兔也表现出LPS诱导的低血压变化。LPS注射后，正常兔的血浆C3水平或血清CH50值仅检测到最小变化或无变化。当通过静脉注射CoF快速激活补体时，兔也会出现低血压变化。与LPS诱导的低血压相反，与补体消耗相关的动脉压下降是短暂的，并且需要大量C3的快速消耗。在注射每种LPS制剂的正常兔中观察到的DIC的发生，在用眼镜蛇因子处理的兔或C6缺陷兔中均未受到抑制。注射Re595和粘质沙雷氏菌LPS后DIC最为明显。注射各种LPS制剂导致循环中的中性粒细胞和单核细胞迅速消失，在正常、CoF处理和C6缺陷的兔中以相同的动力学和相同的程度发生。注射Re595 LPS或粘质沙雷氏菌LPS会导致循环中的51Cr标记血小板出现双相消失。相比之下，注射0111:B4 LPS对51Cr标记血小板的消失速率影响很小。用眼镜蛇因子处理耗尽C3对注射0111:B4的动物中血小板的消失没有影响。与之形成鲜明对比的是，眼镜蛇因子处理大大减少了注射Re595或粘质沙雷氏菌LPS的兔中血小板最初的快速消失，但对二次消失阶段没有影响。
